今天，癌症是世界上最重要的死因之一，迄今已经在这个领域使用了许多治疗方法。免疫治疗被认为是这门科学中最新的发展之一，它仍在不同癌症和不同抗原形式中进行研究。癌症免疫治疗的一个子集是其使用寄生虫抗原来治疗的一种方法。这项研究评估了应用棘球蚴体感抗原对K562癌细胞的效果。在这项研究中，提取了囊肿蚴体感抗原，将其分别添加到K562癌细胞中，分别在三个浓度（0.1、1、2mg/ml）和三个时间（24、48、72小时）下，与对照瓶中的细胞进行比较，观察凋亡细胞的数量。同时，使用浓度为2mg/ml的抗原做为控制样本，研究其对健康HFF3细胞生长的细胞毒性作用。此外，还进行了Annexin V和PI测试，以区分凋亡和坏死。在接受棘球囊蚴体感抗原处理的瓶中，三个浓度均显著降低了癌细胞的生长，且粗抗原的浓度2显著导致癌细胞死亡。此外，随着接触抗原时间的增加，更多的癌细胞经历了凋亡。另一方面，流式细胞术的结果也显示，与对照组相比，凋亡的数量增加了。实际上，棘球囊蚴体感抗原引发了K562癌细胞中的程序性细胞死亡，而对正常细胞没有细胞毒性作用。因此，建议对这种寄生虫抗原的抗癌和治疗特性进行更多的研究。©2023年。作者（们）独家许可给波兰科学院寄生物研究所Witold Stefański研究所。

Today, cancer is one of the most important causes of death in the world, and so far, many treatment methods have been used in this field. Immunotherapy is considered one of the newest developments in this science, and it is still being investigated in some forms in different cancers and with a variety of antigens as well. One of the subsets of cancer immunotherapy is its treatment using parasitic antigens. The present study evaluated the effect of using somatic antigens of protoscoleces of Echinococcus granulosus on K562 cancer cells.In this study, hydatid cysts' protoscolex antigens were extracted, purified, and added to K562 cancer cells at three concentrations (0.1, 1, and 2 mg/ml) and on three times (24, 48, and 72 h). The number of apoptotic cells was compared to the control flask. The antigen concentration of 2 mg/ml was used as a control sample to investigate its cytotoxic effect on the growth of healthy HFF3 cells. Annexin V and PI tests were also performed to differentiate apoptosis from necrosis.In flasks treated with hydatid cyst protoscolex antigen, all three concentrations significantly reduced the growth of cancer cells compared with the control flask, and concentration 2 of crude antigen significantly caused the death of cancer cells. Furthermore, more cancer cells underwent apoptosis by increasing the time of exposure to the antigen. On the other hand, flow cytometry results also showed that the amount of apoptosis has increased compared to the control group. In fact, Protoscolex hydatid cyst somatic antigens induce programmed cell death in K562 cancer cells while not having a cytotoxic effect on normal cells.Therefore, it is suggested to do more research on the anti-cancer and therapeutic properties of the antigens of this parasite.© 2023. The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences.