结肠直肠癌是全球第三致命和第四常见的癌症。芦丁酸是羟基肉桂酸的衍生物，是一种具有许多药理活性的有前途的植物化学物质。它是一种重要的链断裂抗氧化剂，可作为自由基清除剂。本研究旨在研究芦丁酸对HT-29细胞系的抗增殖作用以及其机制。使用XTT法研究芦丁酸对HT-29细胞系存活率的影响。使用ELISA法测量BCL-2、裂解的caspase 3、BAX、裂解的PARP和8-oxo-dG的水平。半定量评估γ-H2AX和细胞色素C的表达，使用免疫荧光染色法。芦丁酸在200 μM及更高剂量时对HT-29细胞产生了显着的抗增殖作用，24小时的IC50值为317.5 μM。芦丁酸（317.5 μM）显著提高了裂解的caspase 3、BAX、裂解的PARP和8-oxo-dG的水平。芦丁酸处理的HT-29细胞中γ-H2AX灶的水平显著升高，而细胞色素C的水平降低。这些结果表明芦丁酸对结肠癌细胞具有抗增殖、凋亡和基因毒性效应。

Colorectal cancer is the third most lethal and fourth most commonly diagnosed cancer worldwide. Sinapic acid, a derivative of hydroxycinnamic acid, is a promising phytochemical exhibiting numerous pharmacological activities in various systems. It is a substantial chain-breaking antioxidant that operates as a radical scavenger. The aim of this research was to investigate the antiproliferative effect of sinapic acid on the HT-29 cell line, besides the mechanisms underlying this activity. The effect of sinapic acid on the viability of HT-29 cell line was investigated using XTT assay. the levels of BCL-2, cleaved caspase 3, BAX, cleaved PARP and 8-oxo-dG were measured using ELISA. Gamma-H2AX and cytochrome C expression was assessed semi-quantitatively using immunofluorescence staining. Sinapic acid at 200 μM and higher doses produced a significant antiproliferative effect on HT-29 cells. The IC50 value was found to be 317.5 μM for 24 hours. Sinapic acid (317.5 μM) significantly elevated cleaved caspase 3, BAX, cleaved PARP and 8-oxo-dG levels. the levels of γ-H2AX foci are significantly higher while the levels of cytochrome-C are lower in sinapic acid treated HT-29 cells. These results indicate that sinapic acid has an antiproliferative, apoptotic and genotoxic effect on colon cancer cells.