lncRNA的去调控已在人类骨肉瘤中观察到。本研究探讨了EPB41L4A-AS1和UNC5B-AS1在骨肉瘤中的诊断和预后意义。在骨肉瘤组织样本和细胞中检测了EPB41L4A-AS1和UNC5B-AS1的相对水平，并通过接收操作特征（ROC）曲线构建评估了区分骨肉瘤和健康的能力。通过Kaplan-Meier（K-M）和Cox比例危险分析进行预后因素评估，使用生物信息学方法鉴定EPB41L4A-AS1和UNC5B-AS1的靶向miRNA。进行Kaplan-Meier生存曲线和Whitney Mann U检验以验证统计学意义。在细胞培养实验中，通过CCK-8和Transwell实验检查了EPB41L4A-AS1和UNC5B-AS1对骨肉瘤细胞系增殖、迁移和侵袭的影响。与健康参与者和正常细胞系相比，骨肉瘤患者和细胞中EPB41L4A-AS1和UNC5B-AS1的水平上调。EPB41L4A-AS1和UNC5B-AS1具有强大的区分患有骨肉瘤的患者和健康的潜力。EPB41L4A-AS1和UNC5B-AS1水平与SSS阶段相关。EPB41L4A-AS1和UNC5B-AS1水平高的患者生存时间显着缩短。EPB41L4A-AS1和UNC5B-AS1是总生存的独立预后指标。miR-1306-5p是EPB41L4A-AS1和UNC5B-AS1的共同靶标。观察到EPB41L4A-AS1和UNC5B-AS1对细胞增殖、迁移和侵袭具有促进作用，但可以通过miR-1306-5p得到救治。结论为EPB41L4A-AS1和UNC5B-AS1表达的上调是人类骨肉瘤的诊断和预后生物标记物。EPB41L4A-AS1和UNC5B-AS1通过miR-1306-5p对骨肉瘤的生物学行为做出贡献。 ©2023年作者。

Deregulation of lncRNAs has been observed in human osteosarcoma. This study explored the diagnostic and prognostic significance of EPB41L4A-AS1 and UNC5B-AS1 in osteosarcoma.Relative levels of EPB41L4A-AS1 and UNC5B-AS1 were detected in osteosarcoma tissue samples and cells. The ability to distinguish osteosarcoma from health was assessed by receiver operating characteristic (ROC) curve construction. Kaplan-Meier (K-M) and Cox proportional-hazards analyses were performed for prognosis factors. The bioinformatics approach was used to identify targeting miRNA for EPB41L4A-AS1 and UNC5B-AS1. Kaplan-Meier survival curves and Whitney Mann U tests were conducted for validating the statistical significance. In cell culture experiments, the influence of EPB41L4A-AS1 and UNC5B-AS1 on proliferation, migration, and invasion of the osteosarcoma cell line was examined by CCK-8 and Transwell assays.Levels of EPB41L4A-AS1 and UNC5B-AS1 were upregulated in osteosarcoma patients and cells compared with the healthy participants and normal cell lines. EPB41L4A-AS1 and UNC5B-AS1 have a potent ability to distinguish the patients with osteosarcoma from the health. EPB41L4A-AS1 and UNC5B-AS1 levels correlated with SSS stage. Patients with high levels of EPB41L4A-AS1 and UNC5B-AS1 had significantly shorter survival times. EPB41L4A-AS1 and UNC5B-AS1 were independent prognostic indexes for overall survival. miR-1306-5p was a common target for EPB41L4A-AS1 and UNC5B-AS1. A propulsive impact on cell proliferation, migration, and invasion by EPB41L4A-AS1 and UNC5B-AS1 was observed, but can be rescued by miR-1306-5p.It was concluded that upregulations of EPB41L4A-AS1 and UNC5B-AS1 expression were diagnostic and prognostic biomarkers for human osteosarcoma. EPB41L4A-AS1 and UNC5B-AS1 contribute to the biological behavior of osteosarcoma via miR-1306-5p.© 2023. The Author(s).