协同化疗已经在临床实践中被证明是一种有效的抗肿瘤手段。然而，大多数联合治疗通常缺乏对不同化疗药物释放的同时控制。β-环糊精改性透明质酸是“外壳”，氧化亚铁茂-十八烷醇胺微粒是“内核”，去氧表雄（DOX）和姜黄素（CUR）分别被加载到双层纳米粒子（BNs）的外壳和内核中。在不同介质中评估pH和谷胱甘肽（GSH）响应的同步释放行为，并进一步研究了体外和体内的协同抗肿瘤效应和CD44介导的肿瘤靶向效率。这些BNs具有球形结构，粒径为299±15.17 nm，两种药物的同步释放行为在pH值为5.5和20 mM GSH的介质中被证明。DOX和CUR的共同传递使IC50值比单独使用DOX降低了21％，在这些BNs的传递测量后进一步降低了54％。在肿瘤负荷的小鼠模型中，这些药物负载的BNs显示出显着的肿瘤靶向，增强的抗肿瘤活性和减少的系统副作用。设计的双层纳米粒子可以被视为潜在的化疗共同递送平台，以实现高效的同步微环境响应和药物释放。此外，同时和协同的药物释放保证了联合治疗期间增强的抗肿瘤效果。© 2023 Zeng 等

Synergistic chemotherapy has been proved as an effective antitumor means in clinical practice. However, most co-administration treatment often lacks simultaneous control over the release of different chemotherapeutic agents.β-cyclodextrin modified hyaluronic acid was the "shell", and the oxidized ferrocene-stearyl alcohol micelles served as the "core", where doxorubicin (DOX) and curcumin (CUR) were loaded in shell and core of the bilayer nanoparticles (BNs), respectively. The pH- and glutathione (GSH)-responsive synchronized release behavior was evaluated in different mediums, and the in vitro and in vivo synergistic antitumor effect and CD44-mediated tumor targeting efficiency were further investigated.These BNs had a spherical structure with the particle size of 299 ± 15.17 nm, while the synchronized release behaviour of those two drugs was proved in the medium with the pH value of 5.5 and 20 mM GSH. The co-delivery of DOX and CUR reduced the IC50 value by 21% compared to DOX alone, with a further 54% reduction after these BNs delivery measurements. In tumor-bearing mouse models, these drug-loaded BNs showed significant tumor targeting, enhanced antitumor activity and reduced systemic toxicity.The designed bilayer nanoparticle could be considered as potential chemotherapeutic co-delivery platform for efficient synchronized microenvironment respond and drug release. Furthermore, the simultaneous and synergistic drug release guaranteed the enhanced antitumor effects during the co-administration treatment.© 2023 Zeng et al.