探究从安泰蚕茧中制备的丝胶制剂的抗癌活性。尽管癌症研究取得了许多进展，但全球的癌症负担仍然显著且不断增加。丝胶是蚕茧中的黏合蛋白，已被证明在各种生物医学应用中具有潜在的蛋白质，包括癌症治疗。本研究评估了来自黄叶蚕安泰蚕茧中的丝胶（SAP）对人类肺癌（A549）和宫颈癌（HeLa）细胞系的抗癌特性。这是首次报道非桑蚕家蚕安泰蚕茧的抗癌活性。确立SAP的抗增殖潜力。2.确定SAP在两种不同细胞系中诱导细胞死亡的分子机制。SAP通过脱胶法从黄叶蚕安泰蚕茧中制备。细胞毒性活性通过MTT测定，基因毒性活性通过彗星试验测定。通过Western印迹分析卡式酶和PARP蛋白的裂解和MAPK途径成员的磷酸化。细胞周期分析通过流式细胞仪完成。SAP分别引起A549和HeLa细胞系的细胞毒性，其IC50值分别为3.8和3.9µg/µl。SAP通过在A549和HeLa细胞中的caspase-3和p38 MAPK途径，以剂量依赖方式诱导细胞凋亡。此外，在A549和HeLa细胞中，SAP通过剂量依赖方式在S期诱导细胞周期阻滞。SAP在A549和HeLa细胞系中诱导的凋亡分子机制的差异可能是由于癌细胞系的基因型差异造成的。然而，还需要进一步的研究。本研究的整体结果预示着使用SAP作为抗肿瘤药物的可能性。版权所有© Bentham Science Publishers；如有任何查询，请发送电子邮件至epub@benthamscience.net。

To investigate the anticancer activity of sericin preparation from cocoons of A. proylei.In spite of much progress in cancer, the global cancer burden is still significant and increasing. Sericin, an adhesive protein of silk cocoons, has been shown to be a potential protein in various biomedical applications, including cancer therapeutics. The present study evaluates the anticancer property of sericin from cocoons of Antheraea proylei J (SAP) against human lung cancer (A549) and cervical cancer (HeLa) cell lines. This is the first report of anti-cancer activity of the non-mulberry silkworm A. proylei J.Establish the antiproliferative potential of SAP. 2. Identify the molecular mechanism of cell death induced by SAP on two different cell lines.SAP was prepared from cocoons of A. proylei J. by the process of the degumming method. Cytotoxicity activity was assessed by MTT assay, and genotoxicity activity was assessed by comet assay. Cleavage of caspase and PARP proteins and phosphorylation of MAPK pathway members were analysed by Western blotting. Cell cycle analysis was done by flow cytometer.SAP causes cytotoxicity to A549 and HeLa cell lines with the IC50 values 3.8 and 3.9 µg/µl respectively. SAP induces apoptosis in a dose-dependent manner through caspase-3 and p38, MAPK pathways in A549 and HeLa cells. Moreover, in A549 and HeLa cells, SAP induces cell cycle arrest at the S phase in a dose-dependent manner.The difference in the molecular mechanisms of apoptosis induced by SAP in A549 and HeLa cell lines may be due to the difference in the genotypes of the cancer cell lines. However, further investigation is warranted. The overall results of the present study envisage the possibility of using SAP as an anti-tumorigenic agent.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.