胃癌是消化系统中常见的癌症。长链非编码RNA（lncRNA）在胃癌的形成和发展中发挥重要作用。本研究旨在研究长链非编码RNA114227对胃癌细胞生物行为的影响。实验分为4组：阴性对照（NC）组、lncRNA114227小干扰（si-lncRNA114227）组、空载体（Vector）组和过表达载体（OE-lncRNA114227）组。通过实时荧光定量PCR（real-time RT-PCR）测定了胃粘膜和胃癌组织、胃粘膜上皮细胞和不同胃癌株中的lncRNA114227表达水平。通过CCK-8检测法检测了胃癌细胞增殖率。Transwell实验、划痕愈合实验和Western blotting实验皆应用EMT技术测定了胃癌细胞的上皮间充质转化能力。通过裸鼠体内肿瘤携带实验检测了lncRNA114227对胃癌细胞增殖的影响。结果表明，在胃癌组织中，lncRNA114227的表达水平显著低于胃粘膜组织，在4种胃癌株中也均显著低于胃粘膜上皮细胞（P <0.01）。在体外实验中，经过表达lncRNA114227后，胃癌细胞的增殖和迁移能力明显降低，而经过沉默lncRNA114227后，细胞增殖和迁移能力增强（P <0.05）。裸鼠皮下肿瘤发生实验的结果显示，携带OE-lncRNA114227群组的肿瘤小鼠的肿瘤体积显著小于Vector组的肿瘤体积，并且肿瘤形态较差（P < 0.05），表明lncRNA114227抑制了肿瘤形成。lncRNA114227的表达在胃癌组织和细胞系中均有下调。lncRNA114227可能通过EMT过程抑制胃癌细胞的增殖和迁移。

Gastric cancer is a common cancer of the digestive system. Long non-coding RNA (lncRNA) plays an important role in the formation and development of gastric cancer. This study aims to investigate the effect of long non-coding lncRNA 114227 on biologic behaviors in gastric cancer cells.The experiment was divided into 4 groups: a negative control (NC) group, a lncRNA 114227 small interference (si-lncRNA 114227) group, an empty vector (Vector) group, and an overexpression vector (OE-lncRNA 114227) group. The expressions of lncRNA 114227 in gastric mucosa and gastric cancer tissues, gastric mucosal epithelial cells and different gastric cancer strains were determined by real-time reverse transcription PCR (real-time RT-PCR).The proliferation were detected by CCK-8 assay in gastric cancer cells. The epithelial-mesenchymal transformation (EMT) was utilized by Transwell assay, scratch healing assay, and Western blotting in gastric cancer cells. The effect of lncRNA 114227 on proliferation of gastric cancer cells was detected by tumor bearing experiment in nude mice in vivo.The expression level of lncRNA 114227 in the gastric cancer tissues was significantly lower than that in the gastric mucosa tissues, and in 4 kinds of gastric cancer strains was all significantly lower than that in gastric mucosal epithelial cells (all P<0.01). In vitro, the proliferation and migration abilities of gastric cells were significantly reduced after overexpressing lncRNA 114227, and cell proliferation and migration were enhanced after silencing lncRNA 114227 (all P<0.05). The results of in vivo subcutaneous tumorigenesis in nude mice showed that the tumorigenic volume of the tumor-bearing mice in the OE-lncRNA 114227 group was significantly smaller than that of the Vector group, and the tumorigenic quality was lower than that of the Vector group (P<0.05), indicating that lncRNA 114227 inhibited tumorigenesis.The expression of lncRNA 114227 is downregulated in gastric cancer gastric cancer tissues and cell lines. LncRNA 114227 may inhibit the proliferation and migration of gastric cancer cells through EMT process.