喉鳞状细胞癌（LSCC）是头颈部常见的恶性肿瘤之一。恶性肿瘤治疗的靶基因筛选是癌症研究的重点之一，原癌基因和肿瘤抑制基因是其中的突破点。寻找与LSCC治疗和预后相关的靶基因已成为紧迫的需求。该研究旨在通过检测这两种蛋白质的表达并分析Lin28B和C-myc在LSCC中的作用，以及它们与LSCC的临床病理特征和预后之间的相关性。我们通过免疫组化检测了102个LSCC标本和90个相邻组织的Lin28B和C-myc蛋白质的表达，分析了LSCC中Lin28B和C-myc蛋白质表达之间的相关性，以及这两种蛋白质的表达与LSCC的临床病理特征之间的关系。同时，使用Kaplan-Meier方法分析了Lin28B和C-myc蛋白水平与LSCC患者术后生存率之间的关系。LSCC组织中的Lin28B和C-myc蛋白水平显著高于相邻组织（P <0.05），并且LSCC中Lin28B和C-myc表达呈正相关（r = 0.476，P <0.05）。 Lin28B蛋白的表达与LSCC患者的年龄、淋巴结转移、临床分期、肿瘤大小和病理分化密切相关（所有P <0.05），而C-myc蛋白的表达与LSCC患者的淋巴结转移、临床分期、肿瘤大小和病理分化密切相关（所有P <0.05）。相应的生存分析显示，在Lin28B（P = 0.001）或C-myc蛋白（P <0.001）水平较高的患者中，术后生存率相对较低。Lin28B和C-myc蛋白在LSCC中高表达并呈正相关。此外，它们与淋巴结转移、临床分期、肿瘤大小、病理分化和预后密切相关，表明Lin28B和C-myc可能参与LSCC的发生和发展。

Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of head and neck. Screening of target genes for malignant tumor therapy is one of the focuses of cancer research, with proto-oncogene and tumor suppressor gene as the breakthrough. It has become an urgent need to find the target gene related to the treatment and prognosis of LSCC.This study aims to explore the role of Lin28B and C-myc in LSCC by detecting the expressions of these two proteins and analyze the correlation between the expression of Lin28B and C-myc and clinicopathological features and prognosis of LSCC.We detected the expression of Lin28B and C-myc proteins in 102 specimens of LSCC and 90 specimens of adjacent tissues by immunochemistry, and analyzed the correlation between Lin28B and C-myc protein expressions in LSCC as well as the correlation between the expressions of the two proteins and the clinicopathological features of LSCC. At the same time, the Kaplan-Meier method was used to analyze the relation between Lin28B and C-myc protein levels with the postoperative survival rate of LSCC patients.The protein levels of Lin28B and C-myc in the LSCC tissnes were significantly higher than those in the adjacent tissues (both P<0.05),and there was a positive correlation between the expression of Lin28B and C-myc in LSCC (r＝0.476, P<0.05). The expression of Lin28B protein was closely related to age, lymph node metastasis, clinical stage, tumor size, and pathological differentiation of LSCC patients (all P<0.05). while the expression of C-myc protein was closely related to lymph node metastasis, clinical stage, tumor size, and pathological differentiation of LSCC patients (all P<0.05). A relevant survival analysis showed that in patients with higher level of Lin28B (P＝0.001) or C-myc protein (P<0.001), the postoperative survival rate was relatively low.Lin28B and C-myc proteins are highly expressed in LSCC with a positive correlation. Furthermore, they are closely related to lymph node metastasis, clinical stage, tumor size, pathological differentiation and prognosis, suggesting that both Lin28B and C-myc might be involved in the occurrence and development of LSCC.