金属蛋白酶-3（MMP3）是软骨退化中涉及的主要酶。多种遗传和非遗传因素可增加骨关节炎（OA）患者中MMP3的表达。本研究旨在分析与MMP3基因rs679620流体滑液膝OA患者表达相关的风险因素。在廖·阿里芬·哈里翁省骨科门诊和佩坎巴鲁市伊本·西那医院进行了横断面研究。通过连续抽样，取90名患有膝OA的女性为样本，并签署知情同意书。数据通过采访使用有关特征的问卷，随后进行体重和身高测量获得。使用酶联免疫吸附法（ELISA）方法从滑液中检测白细胞介素-1β（IL-1β）和肿瘤坏死因子（TNF-α）。通过联合生物医学实验室的关节液DNA分析获得了金属蛋白酶-3（MMP3）基因多态性rs679620。数据经计算机处理，然后使用相关的Spearman-Rank和卡方检验加以分析。统计分析结果的p值为0.05时，认为具有显著性。突变型MMP3 rs679620基因多态性为88.9％，AG和GG等位基因比例相同（44.4％）。年龄≥60岁的受试者占53.3％，85.6％不工作，84.4％已进入绝经期。最高OA程度为2级（53.3％），大部分人具有危险的营养状况（84.4％）。 MMP3 rs679620基因的中位数表达为5.28拷贝数。 MMP3基因多态性rs679620、年龄、IL-1β和TNF-α水平与MMP3基因表达rs679620之间存在显著关系。BMI、工作状态和绝经状态与MMP3基因表达rs679620之间没有显著关系。结论。 MMP3基因多态性rs679620，年龄，IL-1β和TNF-α水平是女性膝OA增加MMP3基因rs679620表达的危险因素。版权：©2023 Sulastri等。本文是按照知识共享署名许可协议发布的开放获取文章，只要原作者和出处被署名，便可在任何媒介中自由传播、使用和复制。

Metalloproteinases-3 (MMP3) are the main enzymes involved in cartilage degradation. Several genetic and non-genetic factors can increase the expression of MMP3 in patients with osteoarthritis (OA). This study aims to analyze the risk factors associated with the expression of the MMP3 gene rs679620 fluid synovial knee OA patients.A cross-sectional study was conducted at the orthopedic polyclinic Arifin Achmad Riau Province and Ibn Sina Hospital in Pekanbaru City. Ninety women who experienced knee OA were taken as samples by consecutive sampling and then signed the informed consent. Data were obtained through interviews using a questionnaire about characteristics, followed by weight and height measurements. Interleukin-1 β (IL-1β) and Tumor Necrosis Factor (TNF-α) were examined from the synovial fluid using the enzyme-linked immunosorbent assay (ELISA) method. The Metalloproteinases-3 (MMP3) gene polymorphism rs679620 was obtained from the DNA analysis of joint fluid results in the Biomedical Laboratory of the Faculty of Medicine, Andalas University. The data was processed computerized and then analyzed using the correlation Spearman-Rank, and chi-square tests. The results of statistical analysis are considered significant if the p-value is 0.05.The MMP3 rs679620 gene polymorphism of the mutant type was 88.9%, with the same proportion of AG and GG alleles (44.4%). Subjects aged ≥ 60 years were 53.3%, 85.6% did not work and 84.4% had menopause. The highest degree of OA was grade 2 (53.3%), most of whom had a risky nutritional status (84.4%). The median expression of the MMP3 rs679620 gene was 5.28 copies number. There is a significant relationship between MMP3 gene polymorphism rs679620, age, IL-1β, and TNF-α with MMP3 gene expression rs679620. There is no significant relationship between BMI, work status, and menopausal status with MMP3 gene expression rs679620. Conclusion. MMP3 gene polymorphism rs679620, age, levels of IL-1β and TNF-α are risk factors for increased MMP3 gene rs679620 expression in female knee OA.Copyright: © 2023 Sulastri et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.