在这项研究中，采用电纺法制备了富含杏仁苦苷（AMG）和苦杏仁子提取物的PLA/PEG纳米纤维（NFs），以预防局部乳腺癌复发，并在体外研究了所制备的NFs对MCF-7细胞系的影响。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、热分析（DSC）等进行了NFs的表征，并进行了拉伸强度和物理分析。将AMG加载到纳米纤维中，使得纤维直径从827.93 ± 174.507 nm增加到1855.32 ± 291.057 μm。药物释放结果分析表明，NFs呈现出延长至10个小时的控制释放曲线。AMG载药NFs的封装效率分别为100 ± 0.01%、94 ± 0.02%和88 ± 0.02%。细胞毒性实验结果表明，所有NFs均能有效诱导MCF-7乳腺癌细胞的细胞毒性。尤其是20mg的AMG载药NFs，对乳腺癌细胞具有更高的细胞毒性效应。综合考虑，AMG载药NFs可以在局部部位持续释放药物，减少手术后癌症局部复发的风险，并可直接植入固体肿瘤细胞治疗。版权所有©2023 Elsevier B.V.出版

In this study, PLA/PEG nanofibers (NFs) loaded with amygdalin (AMG) and bitter almond kernels extract were produced by electrospinning to prevent local breast cancer recurrence, and the effect of produced NFs on the MCF-7 cell line was investigated in vitro. The electrospun NFs were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), thermal analysis (DSC) and tensile strength and physical analyzes were performed. Loading of AMG to nanofibers increased fiber diameters from 827.93 ± 174.507 nm to 1855.32 ± 291.057 μm. When drug release results were analyzed, the NFs showed a controlled release profile extending up to 10 h. The encapsulation efficiency of AMG-loaded NFs was calculated at 100 ± 0,01 %, 94 ± 0,02 %, and 88 ± 0,02 %. When in vitro cytotoxicity results were analyzed, showed that all NFs are effective in inducing cytotoxicity against MCF-7 breast cancer cells. Importantly, 20 mg AMG-loaded NFs displayed effectively higher cytotoxic effects against breast cancer cells relative to the other NFs. Considering all the results, AMG-loaded NFs can give sustained release of drugs at the local sites. Therefore, AMG-loaded nanofibers can reduce the risk of local recurrence of cancer after surgery and can be directly implanted into solid tumor cells for treatment.Copyright © 2023. Published by Elsevier B.V.