细胞外囊泡（EVs）是从细胞中释放的颗粒，它们的脂质双层膜包裹着大量的生物活性分子，赋予EVs细胞间或组织间的通讯能力。组织源性细胞外囊泡（Ti-EVs）是直接从组织间隙分离出的EVs。与细胞系来源的EVs和生理液体EVs相比，它们能更好地反映组织微环境的实际生理或病理状态，表明它们在阐明发病机制、指导疾病诊断、治疗靶向和无细胞治疗方面具有潜在作用。然而，对Ti-EVs的研究还相对有限。在本文中，我们总结了Ti-EVs分离的一般程序，以及随后操作（例如纯化和储存）时的一些注意事项。此外，我们还简要总结了来自各种正常和肿瘤组织的EVs的当前研究趋势，旨在为Ti-EVs未来的研究方向提供新的思路。版权所有©2023 Elsevier Inc.发布。

Extracellular vesicles (EVs) are particles released from cells, and their lipid bilayer membrane encloses large amounts of bioactive molecules that endow EVs with intercellular or inter-tissue communicational abilities. Tissue-derived extracellular vesicles (Ti-EVs) are EVs directly separated from the interstitial space of tissue. They could better reflect the actual physiological or pathological state of the tissue microenvironment compared with cell line-derived EVs and biofluid EVs, indicating their potential roles in elucidating the underlying mechanism of pathogenesis and guiding the diagnosis, therapeutic targeting, and cell-free treatment of diseases. However, there have been a relatively limited number of investigations of Ti-EVs. In this review, we have summarized general procedures for Ti-EVs isolation, as well as some caveats with respect to operations after the isolation step, such as purification and storage. In addition, we have also briefly concluded the current research trends on EVs from various normal and tumor tissues, aiming to cast new light on the future research direction of Ti-EVs.Copyright © 2023. Published by Elsevier Inc.