光疗法是一种新的治疗肿瘤的方法，包括光动力疗法（PDT）和光热疗法（PTT）。然而，肿瘤细胞中的谷胱甘肽（GSH）可能会耗尽光敏剂产生的ROS，导致PDT效果不佳。异硫氰酸盐不仅是一种新型抗肿瘤药物，而且还可以与GSH结合，增加细胞内ROS含量，提高PDT效果。在此，我们合成了一种水溶性纳米颗粒（BN NPs），用mPEG-ITC和磷脂包含BODIPY-I-35。mPEG-ITC可以与肿瘤细胞中的GSH反应，减少ROS的消耗。BN NPs可作为载药向肿瘤部位输送药物。在808nm激光照射下，BN NPs溶液在10分钟内升高了13℃，表明BN NPs具有出色的光热性能。体外实验中，低剂量的BN NPs显示出满意的PDT和PTT效果，MCF-7细胞的细胞存活率仅为13％。在体内，具有优异生物相容性的BN NPs显示出良好的光疗效果，并有效抑制了肿瘤。荧光成像可以展示BN NPs在肿瘤部位的长期滞留效果。总之，BN NPs显示了增强光疗效果并在肿瘤细胞光疗中提供了显着的应用前景。版权所有©2023 Elsevier B.V.。保留所有权利。

Phototherapy is a new method to treat tumor, including photodynamic therapy (PDT) and photothermal therapy (PTT). However, the GSH in tumor cells could deplete ROS produced by photosensitizers, resulting in inadequate PDT. Isothiocyanate not only is a new type of anti-tumor drug, but also may combine with GSH, increasing the content of intracellular ROS and improving PDT effect. Here we synthesized a kind of water-soluble nanoparticles (BN NPs) parceling BODIPY-I-35 up with mPEG-ITC and lecithin. mPEG-ITC can react with GSH in tumor cells to reduce the consumption of ROS. BN NPs can be used as vectors to deliver drugs to tumor sites. Under 808 nm laser irradiation, BN NPs solution increased 13 °C within 10 min, indicating that BN NPs had superb photothermal performance. In vitro experiments, low dose BN NPs showed satisfactory PDT and PTT effects, and the cell viability of MCF-7 cell was only 13%. In vivo, BN NPs with excellent biocompatibility showed favorable phototherapy effect and tumor was effectively inhibited. Fluorescence imaging could present the long retention effect of BN NPs in tumor locations. In conclusion, the BN NPs showed the effect of enhancing phototherapy and provided a remarkable application prospect in the phototherapy of tumor cells.Copyright © 2023 Elsevier B.V. All rights reserved.