使用人胰腺癌细胞和人工iPSC衍生的星形细胞开发一种新型共培养系统来模拟体外胰管腺癌的特征。
Development of a novel co-culture system using human pancreatic cancer cells and human iPSC-derived stellate cells to mimic the characteristics of pancreatic ductal adenocarcinoma in vitro.
发表日期：2023 Mar 24
作者：
Tatsuya Kometani, Koki Kamo, Taketomo Kido, Nobuyoshi Hiraoka, Taku Chibazakura, Kenji Unno, Keisuke Sekine
来源：
Stem Cell Research & Therapy
摘要：
胰管腺癌（PDAC）是一种预后不良和易于化疗抵抗的严重疾病。据推测，PDAC的肿瘤微环境（TME）对这些特征有贡献。然而，胰腺癌细胞与TME中的基质细胞之间的详细作用机制尚不清楚。因此，本研究的目的是建立一个共培养系统，模拟TME，使用来自PDAC患者标本的癌细胞和人类诱导多能干细胞的星形细胞作为基质细胞。我们成功观察到了癌细胞与星形细胞之间的相互作用，并在我们的共培养系统中重现了一些PDAC的特征。此外，我们还证明了我们的共培养系统在体外药物治疗方面的适用性。总之，我们提出我们的共培养系统作为一种新的方法来分析细胞间相互作用，特别是在PDAC的TME中。© 2023 Elsevier Inc. All rights reserved.
Pancreatic ductal adenocarcinoma (PDAC) is a serious disease with poor prognosis and prone to chemotherapy resistance. It is speculated that the tumor microenvironment (TME) of PDAC contributes to these characteristics. However, the detailed mechanisms of interactions between pancreatic cancer cells and stroma in the TME are unclear. Therefore, the aim of this study was to establish a co-culture system that mimics the TME, using cancer cells derived from PDAC patient specimens and stellate cells from human induced pluripotent stem cells as stromal cells. We succeeded in observing the interaction between cancer cells and stellate cells and reproduced some features of PDAC in vitro using our co-culture systems. In addition, we demonstrated the applicability of our co-culture system for drug treatment in vitro. To conclude, we propose our co-culture system as a novel method to analyze cell-cell interactions, especially in the TME of PDAC.Copyright © 2023 Elsevier Inc. All rights reserved.