我们的目的是研究激动脂多糖（LPS）后通过地塞米松或地塞米松加雷帕霉素治疗获得的CD14++CD16+单核细胞中白细胞介素（IL）-10、IL-1β、IL-6和肿瘤坏死因子（TNF）-α表达在临床类固醇反应者（R）和不反应者（NR）之间的差异。使用流式细胞术确定了R和NR的LPS刺激CD14++CD16+ p哺乳动物雷帕霉素靶蛋白（mTOR）单核细胞的细胞因子表达。尽管在NR组中减少，但接受地塞米松治疗后，IL-10高表达 CD14++CD16+ p-mTOR 族群在R组中增加。尽管在NR组中增加，但IL-1β高表达群体在R组中减少。 LPS和地塞米松之后用雷帕霉素治疗导致NR组中IL-10高表达群体显著增加，而IL-1β高表达群体显著减少。地塞米松治疗导致R和NR之间LPS刺激CD14++CD16+ p-mTOR单核细胞细胞因子表达变化的不同模式。mTOR抑制可以恢复CD14++CD16+ p-mTOR单核细胞中IL-10和IL-1β的类固醇反应性。

We aimed to investigate the differences in interleukin (IL)-10, IL-1β, IL-6, and tumor necrosis factor (TNF)-α expression in lipopolysaccharide (LPS)-stimulated CD14++CD16+ monocytes obtained from asthmatics after dexamethasone or dexamethasone plus rapamycin treatments between clinical steroid responders (R) and non-responders (NR).Cytokine expressions in LPS-stimulated CD14++CD16+ p-mammalian target of rapamycin (mTOR) monocytes from R and NR were determined using flow cytometry.IL-10high CD14++CD16+ p-mTOR population following LPS stimulation increased in the R group although decreased in the NR group with dexamethasone treatment. IL-1βhigh population decreased in the R group although increased in the NR group. Rapamycin treatment after LPS and dexamethasone resulted in a significant increase in the IL-10high population and a significant decrease in the IL-1βhigh population in the NR group.Dexamethasone treatment resulted in different patterns of change in cytokine expressions in LPS-stimulated CD14++CD16+ p-mTOR monocytes between the R and NR. mTOR inhibition can restore steroid responsiveness involving IL-10 and IL-1β in CD14++CD16+ p-mTOR monocytes.