膀胱癌（BLCA）是最致命的疾病之一。COL10A1是外细胞基质中分泌的小链胶原蛋白，与各种肿瘤有关，包括胃癌、结肠癌、乳腺癌和肺癌。然而，COL10A1在BLCA中的作用仍不清楚。这是第一项关注COL10A1在BLCA预后价值的研究。在这项研究中，我们旨在揭示COL10A1与预后以及BLCA中其他临床病理学参数之间的关联。我们从TCGA、GEO和ArrayExpress数据库中获取了BLCA和正常组织的基因表达谱。进行了免疫组化染色以调查COL10A1在BLCA患者中的蛋白表达和预后价值。进行GO和KEGG富集以及GSEA分析，以基因共表达网络为基础揭示COL10A1的生物学功能和潜在调节机制。我们使用“maftools”R包来显示高和低COL10A1群体之间的突变谱。利用GIPIA2、TIMER和CIBERSORT算法探索COL10A1对肿瘤免疫微环境的影响。我们发现COL10A1在BLCA样本中上调，并且增加的COL10A1表达与不良总生存率有关。共表达了与COL10A1表达呈正相关的200个基因的功能注释，包括GO、KEGG和GSEA富集分析，表明COL10A1基本上参与了细胞外基质、蛋白质修饰、分子结合、ECM-受体相互作用、蛋白质消化和吸收、聚焦间质和PI3K-Akt信号通路。高和低COL10A1群体的最常见突变基因不同。肿瘤免疫浸润分析表明，COL10A1可能在招募浸润的免疫细胞和调节BLCA中的免疫方面起着重要作用，从而影响预后。最后，使用外部数据集和生物样品，并进一步验证了COL10A1在BLCA样本中的异常表达。总之，我们的研究证明了COL10A1是BLCA的潜在预后和预测生物标志物。版权所有©2023 Wang, Bai, Zhang, Li, Chen, Wu, Tang, Wei and Han。

Bladder cancer (BLCA) is one of the most lethal diseases. COL10A1 is secreted small-chain collagen in the extracellular matrix associated with various tumors, including gastric, colon, breast, and lung cancer. However, the role of COL10A1 in BLCA remains unclear. This is the first research focusing on the prognostic value of COL10A1 in BLCA. In this research, we aimed to uncover the association between COL10A1 and the prognosis, as well as other clinicopathological parameters in BLCA.We obtained gene expression profiles of BLCA and normal tissues from the TCGA, GEO, and ArrayExpress databases. Immunohistochemistry staining was performed to investigate the protein expression and prognostic value of COL10A1 in BLCA patients. GO and KEGG enrichment along with GSEA analyses were performed to reveal the biological functions and potential regulatory mechanisms of COL10A1 based on the gene co-expression network. We used the "maftools" R package to display the mutation profiles between the high and low COL10A1 groups. GIPIA2, TIMER, and CIBERSORT algorithms were utilized to explore the effect of COL10A1 on the tumor immune microenvironment.We found that COL10A1 was upregulated in the BLCA samples, and increased COL10A1 expression was related to poor overall survival. Functional annotation of 200 co-expressed genes positively correlated with COL10A1 expression, including GO, KEGG, and GSEA enrichment analyses, indicated that COL10A1 was basically involved in the extracellular matrix, protein modification, molecular binding, ECM-receptor interaction, protein digestion and absorption, focal adhesion, and PI3K-Akt signaling pathway. The most commonly mutated genes of BLCA were different between high and low COL10A1 groups. Tumor immune infiltrating analyses showed that COL10A1 might have an essential role in recruiting infiltrating immune cells and regulating immunity in BLCA, thus affecting prognosis. Finally, external datasets and biospecimens were used, and the results further validated the aberrant expression of COL10A1 in BLCA samples.In conclusion, our study demonstrates that COL10A1 is an underlying prognostic and predictive biomarker in BLCA.Copyright © 2023 Wang, Bai, Zhang, Li, Chen, Wu, Tang, Wei and Han.