目的：本研究旨在探讨玻璃化/复苏过程对人卵巢组织mRNA转录组的影响。设计：人卵巢组织经过玻璃化（T组）处理后，接受RNA测序（RNA-seq）分析、HE、TdT介导的dUTP末端标记（TUNEL）、实时定量PCR检测，并与新鲜组（CK组）进行结果比较。结果：共有12名年龄在15-36岁之间、平均反睾酮激素水平为4.57±3.31 ng/mL的患者被纳入研究。根据HE和TUNEL结果，玻璃化有效地保存了人卵巢组织。CK组和T组之间确定452个明显的基因失调（|log2FoldChange| > 1和p < 0.05）。其中，329种上调和123种下调。共有372种基因高度富集于43个通路（p < 0.05），主要与系统性红斑狼疮、细胞因子-细胞因子受体相互作用、TNF信号通路、MAPK信号通路相关。与CK组相比，T组中IL10、AQP7、CCL2、FSTL3和IRF7均显著上调（p < 0.01），而IL1RN、FCGBP、VEGFA、ACTA2和ASPN显著下调（p < 0.05），这与RNA-seq分析的结果相符。结论：这些结果首次表明，玻璃化可诱导人卵巢组织mRNA表达的变化。需要进行进一步的分子研究，以确定改变的基因表达是否会导致任何下游后果。版权所有 © 2023 Gu、Ge、Huang、Fu、Zhang、Wang、Xu、Li、Peng、Zou、Sun和Sun。

Objective: This study investigated the effects of a vitrification/warming procedure on the mRNA transcriptome of human ovarian tissues. Design: Human ovarian tissues were collected and processed through vitrification (T-group) and then subjected to RNA sequencing (RNA-seq) analysis, HE, TdT-mediated dUTP nick-end labeling (TUNEL), and real-time quantitative PCR, and the results were compared to those of the fresh group (CK). Results: A total of 12 patients, aged 15-36 years old, with a mean anti-Müllerian hormone level of 4.57 ± 3.31 ng/mL were enrolled in this study. According to the HE and TUNEL results, vitrification effectively preserved human ovarian tissue. A total of 452 significantly dysregulated genes (|log2FoldChange| > 1 and p < 0.05) were identified between the CK and T groups. Among these, 329 were upregulated and 123 were downregulated. A total of 372 genes were highly enriched for 43 pathways (p < 0.05), which were mainly related to systemic lupus erythematous, cytokine-cytokine receptor interaction, the TNF signaling pathway, and the MAPK signaling pathway. IL10, AQP7, CCL2, FSTL3, and IRF7 were significantly upregulated (p < 0.01), while IL1RN, FCGBP, VEGFA, ACTA2, and ASPN were significantly downregulated in the T-group (p < 0.05) compared to the CK group, which agreed with the results of the RNA-seq analysis. Conclusion: These results showed (for the first time to the authors' knowledge) that vitrification can induce changes in mRNA expression in human ovarian tissues. Further molecular studies on human ovarian tissues are required to determine whether altered gene expression could result in any downstream consequences.Copyright © 2023 Gu, Ge, Huang, Fu, Zhang, Wang, Xu, Li, Peng, Zou, Sun and Sun.