目的：本研究探讨Th1/Th2细胞因子失衡与25-羟基维生素D（维D）在早期慢性阻塞性肺疾病（COPD）中的相关性，为维D在COPD预防和控制中的作用提供实验理论，并阐明可能涉及的抗炎机制。方法：本研究基于深圳市前海蛇口自由贸易区医院开展的“COPD筛查和早期诊断”公共卫生项目的结果。选择早期COPD患者作为研究对象。采用前瞻性、随机、对照的方法将符合条件的参与者分为三组，即COPD肺功能（LF）I组、COPD LF II组和健康组（每组n = 40）。采用酶联免疫吸附法测定血清肿瘤坏死因子α（TNF-α）、干扰素γ（IFN-γ）、白细胞介素4（IL-4）和IL-6的含量，并将IFN-γ/IL-4比值作为Th1/Th2的标记。化学发光法测定血清25-羟基维D（25 [OH]D）的浓度。进行统计处理，检查上述参数与维D水平和LF参数的变化之间的相关性。结果：健康组、COPD LF I组和COPD LF II组之间存在FEV1pred％、FEV1/FVC、IFN-γ、IL-4、IL-6和IFN-γ/IL-4的差异（p <0.05）。在早期COPD中，Th1/Th2细胞因子与用力呼气容积/期望值（FEV1pred％）（r = 0.485，p <0.001）和用力呼气容积/用力肺活量比值（FEV1/FVC）（r = 0.273，p = 0.018）呈正相关；Th1/Th2细胞因子水平与维D水平呈正相关（r = 0.27，p = 0.02），25（OH）D水平与FEV1pred％呈正相关（r = 0.695，p <0.001）。结论：早期COPD患者普遍存在维D缺乏。它与FEV1pred％和FEV1/FVC LF参数呈正相关。因此，本研究为维D在COPD预防和控制中的作用及可能涉及的抗炎机制提供了实验理论依据。版权所有©2023 Tang, Rong, Zhang, Zhan, Yuan, Ning, and Lin.

Objective: This study explored the correlation between a Th1/Th2 cytokines imbalance and 25-hydroxy-vitamin D (vit D) level in early chronic obstructive pulmonary disease (COPD), provided experimental rationales for the role of vit D in the prevention and control of COPD, and elucidated the potential anti-inflammatory mechanism involved. Methods: This study was based on the results of the "Screening and Early Diagnosis of COPD" public health project conducted through Shenzhen Municipal Qianhai Shekou Free Trade Zone Hospital. Patients with early COPD were selected as study participants. A prospective, randomized, and controlled method was employed for assigning eligible participants into three groups, i.e., a COPD lung function (LF) I, COPD LF II, and a healthy group, respectively (n = 40 each). The serum content of tumor necrosis factor alpha (TNF-α), interferon-gamma (IFN-γ), interleukin 4 (IL-4), and IL-6 were measured by enzyme-linked immunosorbent assay, and the ratio of IFN-γ/IL-4 treated as a marker for Th1/Th2. The serum concentration of 25-hydroxyl-vit D (25 [OH]D) was quantified by a chemiluminescence assay. Statistical processing was performed, and the correlations between changes in the above parameters with vit D level and LF parameters were examined. Results: There were differences in FEV1pred%, FEV1/FVC, IFN-γ, IL-4, IL-6 and IFN-γ/IL-4 between the healthy group, the COPD LF I group and the COPD LF II group (p < 0.05). In early COPD, Th1/Th2 cytokines was positively correlated with forced expiratory volume/expected value (FEV1pred%) (r = 0.485, p < 0.001) and forced expiratory volume/forced vital capacity (FEV1/FVC) (r = 0.273, p = 0.018); Th1/Th2 cytokines levels positively correlated with vit D level (r = 0.27, p = 0.02), and 25(OH)D level positively correlated with FEV1pred% (r = 0.695, p < 0.001). Conclusion: Vitamin D deficiency was ubiquitous in patients with early COPD. It was positively correlated with the FEV1pred% and FEV1/FVC LF parameters. Accordingly, this study provides experimental rationales for the role of vit D in the prevention and control of COPD and the potential anti-inflammatory mechanisms involved.Copyright © 2023 Tang, Rong, Zhang, Zhan, Yuan, Ning and Lin.