为了从调节着床窗口开启时间和子宫内膜容受性的角度探讨针灸对改善COH大鼠妊娠率的影响，实验大鼠随机分为正常组(N)、模型组(M)和针灸组(A)，在交配后的第4、5和6天采集样本。 COH大鼠在SP6，LR3和ST36穴位处接受每天一次，共7次的针灸治疗。通过扫描电子显微镜观察到了松果体乳头突。通过ELISA测量血清雌激素和孕激素水平。通过West-blot、免疫组织化学和PCR评估子宫内的雌激素受体（ER）、孕激素受体（PR）、白血病抑制因子（LIF）、整合素β3、血管内皮生长因子（VEGF）和成纤维细胞生长因子2（FGF-2）的蛋白和mRNA水平。 与N组相比，M组的妊娠率显著降低（P<0.05），并观察到异常的血清激素水平和着床窗口提前。 与M组相比，A组的妊娠率显著增加（P<0.05），过高的血清孕激素水平恢复到正常水平（P<0.05），并且提前的着床窗口被在一定程度上恢复。 此外，子宫内膜异常的ER、PR、LIF、整合素β3、VEGF和FGF-2表达水平得到不同程度的恢复。针灸可能在一定程度上恢复COH大鼠的雌激素和孕激素平衡以及着床窗口的前移，改善子宫内膜容受性，最终改善COH大鼠的妊娠率。版权所有 © 2023 Hu，Huang，Song，Wu，Song，Huang，Zhang和Dong。

To investigate the effect of acupuncture for improving the pregnancy rate of COH rats from the viewpoint of regulating the opening time of the implantation window and endometrial receptivity.Experimental rats were randomly divided into normal group (N), model group (M) and acupuncture group(A), and samples were collected on Day 4, 5 and 6 after mating. COH rats were treated with acupuncture at SP6, LR3, and ST36 once a day for 7 times. The pinopodes were observed under a scanning electron microscope. Serum estrogen and progesterone levels were measured via ELISA. The protein and mRNA levels of estrogen receptor (ER), progesterone receptor (PR), leukemia inhibitory factor (LIF), integrin β3, vascular endothelial growth factor (VEGF), and fibroblast growth factor 2 (FGF-2) in the endometrium were evaluated via West-blot, immunohistochemistry, and PCR.Compared with group N, the pregnancy rate of group M was significantly decreased (P<0.05), and the abnormal serum hormone levels and implantation window advancement were observed. Compared with group M, the pregnancy rate of group A was significantly increased (P<0.05), the supraphysiological serum progesterone levels were restored to normalcy (P<0.05), and the advanced implantation window was restored to a certain extent. Further, the abnormal ER, PR, LIF, integrin β3, VEGF, and FGF-2 expression levels of the endometrium got recovered to varying degrees.Acupuncture may restore the estrogen and progesterone balance in COH rats and the forward shift of the implantation window to a certain extent, improving the endometrial receptivity and finally improving the pregnancy rate of COH rats.Copyright © 2023 Hu, Huang, Song, Wu, Song, Huang, Zhang and Dong.