本研究的目的是定位单核细胞趋化蛋白1引起的蛋白1（MCPIP-1）及其抑制剂黏膜相关淋巴组织淋巴瘤易位蛋白1（MALT-1）在龈组织中的分布，并与临床炎症、Porphyromonas gingivalis菌落和白细胞介素(IL)-8水平的蛋白表达水平进行比较。本研究样本来自两个独立的研究人群：（1）从八名牙周健康人和八名牙周病患者中收集龈组织，经免疫组化法定位MCPIP-1和MALT-1；（2）从20名牙周炎患者中收集41份龈组织样本，分别为带状、轻微或中度至重度炎症，使用免疫印迹法确定MCPIP-1和MALT-1水平，qPCR检测P. gingivalis水平，荧光底物测定P. gingivalisgingipain活性，并用多重技术测定IL-8水平。MCPIP-1可在上皮和结缔组织中检测到，在健康牙周组织中特别突出，围绕血管壁。MALT-1观察到在牙龈上皮的所有层次和结缔组织中特别围绕炎症细胞积聚。与牙龈炎症的严重程度相关的龈组织MCPIP-1和MALT-1水平没有差异。MALT-1水平随组织P. gingivalis水平的增加而升高（p = 0.023），MALT-1和IL-8水平存在相关性（β = 0.054，p = 0.001）。MALT-1水平与牙龈组织P. gingivalis计数和IL-8水平的相互作用表明，MALT-1的活化可以参与P. gingivalis调节的宿主免疫反应。通过药理学靶向免疫反应和MCPIP-1/MALT-1之间的相互作用，可能在牙周治疗中产生益处。 © 2023作者。

The purposes of this study were to localize monocyte chemoattractant protein-1-induced protein-1 (MCPIP-1) and its suppressor mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT-1) in gingival tissues and to profile their protein expression levels in relation to the clinical inflammation, Porphyromonas gingivalis colonization, and interleukin (IL)-8 levels.Study samples were collected from two independent study populations: (1) Gingival tissues were collected from eight periodontally healthy individuals and eight periodontitis patients to localize MCPIP-1 and MALT-1 immunohistochemically, and (2) forty-one gingival tissue samples with marginal, mild, or moderate to severe inflammation were collected from 20 periodontitis patients to determine MCPIP-1 and MALT-1 levels using immunoblots, P. gingivalis levels with qPCR, P. gingivalis gingipain activities with fluorogenic substrates, and IL-8 levels with multiplex technique.MCPIP-1 was detectable in the epithelium and in connective tissue, being especially prominent around the blood vessel walls in healthy periodontal tissues. MALT-1 was observed at all layers of gingival epithelium and especially around the accumulated inflammatory cells in connective tissue. No difference in gingival tissue MCPIP-1 and MALT-1 levels was observed in relation to the severity of gingival inflammation. MALT-1 levels were elevated (p = 0.023) with the increase in tissue P. gingivalis levels, and there was an association between MALT-1 and IL-8 levels (β = 0.054, p = 0.001).Interactions of MALT-1 levels with gingival tissue P. gingivalis counts and IL-8 levels suggest that activation of MALT-1 can take part in P. gingivalis-regulated host immune responses.Pharmacological targeting the crosstalk between immune response and MCPIP-1/MALT-1 may have benefits in periodontal treatment.© 2023. The Author(s).