亚撒哈拉地区乳腺癌（BC）患者的免疫景观鲜有研究。我们的目的是描述肿瘤浸润淋巴细胞（TILs）在肿瘤内部基质（sTILs）和前导/侵袭边缘基质（LE-TILs）中的分布，并评估BC亚型中的TILs在肯尼亚女性的风险因素和临床特征上的分布。根据国际TIL工作组的指南，对盖有苏木素和伊红染色的病理证实的BC病例进行了sTIL和LE-TIL的视觉定量。构建了组织芯片阵列，并用免疫组织化学（IHC）的CD3、CD4、CD8、CD68、CD20和FOXP3染色。在调整其他协变量后，使用线性和 logistic 回归模型评估风险因素和肿瘤特征与IHC标记和总TILs之间的关联。共包括226例浸润性BC病例。总体而言，LE-TIL（平均值= 27.9，SD = 24.5）比sTIL（平均值= 13.5，SD = 15.8）比例显著高。sTILs和LE-TILs主要由CD3、CD8和CD68组成。我们发现，TILs较高与高K167/高级别和侵袭性肿瘤亚型相关，尽管这些关联因TIL位置而异。月经初潮年龄较大（≥15岁与<15岁）与较高的CD3相关（OR：2.06，95％CI：1.26-3.37），但仅限于肿瘤内基质。更具侵略性的BC中的TIL富集类似于其他人群中先前发表的数据。sTIL / L E-TIL措施与大多数考虑的因素的不同关联，凸显了未来研究中空间TIL评估的重要性。©2023年。作者(们)专有许可Springer Science+Business Media，LLC，Springer Nature的一部分。

The immune landscape of breast cancer (BC) in patients from Sub Saharan Africa is understudied. Our aims were to describe the distribution of Tumour Infiltrating Lymphocytes (TILs) within the intratumoural stroma (sTILs) and the leading/invasive edge stroma (LE-TILs), and to evaluate TILs across BC subtypes with established risk factors and clinical characteristics in Kenyan women.Visual quantification of sTILs and LE-TILs were performed on Haematoxylin and eosin -stained pathologically confirmed BC cases based on the International TIL working group guidelines. Tissue Microarrays were constructed and stained with immunohistochemistry (IHC) for CD3, CD4, CD8, CD68, CD20, and FOXP3. Linear and logistic regression models were used to assess associations between risk factors and tumour features with IHC markers and total TILs, after adjusting for other covariates.A total of 226 invasive BC cases were included. Overall, LE-TIL (mean = 27.9, SD = 24.5) proportions were significantly higher than sTIL (mean = 13.5, SD = 15.8). Both sTILs and LE- TILs were predominantly composed of CD3, CD8, and CD68. We found higher TILs to be associated with high KI67/high grade and aggressive tumour subtypes, although these associations varied by TIL locations. Older age at menarche (≥ 15 vs. < 15 years) was associated with higher CD3 (OR: 2.06, 95%CI:1.26-3.37), but only for the intra-tumour stroma.The TIL enrichment in more aggressive BCs is similar to previously published data in other populations. The distinct associations of sTIL/LE-TIL measures with most examined factors highlight the importance of spatial TIL evaluations in future studies.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.