基于四氧化三铁（Fe3O4）/羧基化碳纳米管（MWCNTs-COOH）/金纳米颗粒（AuNPs）的电化学生物传感器被设计用于检测甲胎蛋白（AFP），该物质常被用作肝癌标记的早期临床诊断的重要指标。Fe3O4/MWCNTs-COOH纳米复合物通过热溶法合成，并与AuNPs一起沉积在玻碳电极上形成Fe3O4/MWCNTs-COOH/AuNPs，这可以增强电信号，并使AFP单克隆抗体更稳定地固定在电极上。详细研究了Fe3O4/MWCNTs-COOH/AuNPs的电化学性能，并记录了与AFP抗原-抗体的免疫反应后的电化学响应信号。响应信号的峰电流Ip在1pg/mL-10μg/mL范围内与lgcAFP成线性比例关系，检测限为1.09034 pg/mL，且在临床样品测试中表现良好。该传感器在临床医学领域有着广泛应用和发展潜力。版权所有 © 2023 Elsevier B.V.。

An electrochemical biosensor based on iron tetroxide (Fe3O4)/carboxylated carbon nanotubes (MWCNTs-COOH)/gold nanoparticles (AuNPs) was designed for the detection of alpha-fetoprotein (AFP), which is often used as an important indicator for the early clinical diagnosis of liver cancer markers. The Fe3O4/MWCNTs-COOH nanocomposite was synthesized by a solvothermal method and it combined with gold nanoparticles (AuNPs) deposited at the constant potential on a glassy carbon electrode to form Fe3O4/MWCNTs-COOH/AuNPs, which intensifies the electrical signal while the large active sites enable more stable immobilization of the AFP monoclonal antibodies on the electrode. The electrochemical performance of Fe3O4/MWCNTs-COOH/AuNPs was investigated in detail and the electrochemical response signal after the immune reaction with the AFP antigen-antibody was recorded. The peak current Ip of the response signal is linearly proportional to the lgcAFP in the range of 1 pg mL-1-10 μg mL-1, with a detection limit of 1.09034 pg mL-1 and good performance in clinical sample testing. The proposed sensor has shown great application and development potential in clinical medicine field.Copyright © 2023 Elsevier B.V. All rights reserved.