专注于转录本 5'端的单细胞RNA测序方法能够揭示启动子和增强子的活性，并有效地分析免疫受体库。然而，目前还没有描述超高通量的5'端单细胞RNA测序方法。我们介绍了FIPRESCI，即5'端单细胞组合索引RNA-Seq，可以实现大规模样本多重复合，并将液滴微流控系统的吞吐量提高了十倍以上。我们证明，FIPRESCI能够在单通道实验中从E10.5的全小鼠胚胎中生成约10万个单细胞转录组，并同时确定12名癌症患者外周血T细胞的亚群差异和T细胞受体签名。 ©2023年作者。

Single-cell RNA sequencing methods focusing on the 5'-end of transcripts can reveal promoter and enhancer activity and efficiently profile immune receptor repertoire. However, ultra-high-throughput 5'-end single-cell RNA sequencing methods have not been described. We introduce FIPRESCI, 5'-end single-cell combinatorial indexing RNA-Seq, enabling massive sample multiplexing and increasing the throughput of the droplet microfluidics system by over tenfold. We demonstrate FIPRESCI enables the generation of approximately 100,000 single-cell transcriptomes from E10.5 whole mouse embryos in a single-channel experiment, and simultaneous identification of subpopulation differences and T cell receptor signatures of peripheral blood T cells from 12 cancer patients.© 2023. The Author(s).