N7-甲基鸟嘌呤(m7G)甲基化是真核生物中最常见的RNA修饰之一，最近引起了相当大的关注。 m7G修饰在tRNA、rRNA、mRNA和miRNA等RNA中的生物功能在人类疾病中仍然大多不为人知。由于高通量技术的快速发展，越来越多的证据表明m7G修饰在癌症的发生和进展中起着关键作用。由于m7G修饰和癌症的标志密不可分，针对m7G调节因子可能为未来癌症诊断和潜在干预靶点提供新的可能性。本综述总结了m7G修饰的各种检测方法、m7G修饰和肿瘤生物学的最新进展以及它们之间的相互作用和调控机制。最后，我们展望了诊断和治疗m7G相关疾病的未来。版权所有©2023 Elsevier B.V。保留所有权利。

N7-methylguanosine (m7G) methylation, one of the most common RNA modifications in eukaryotes, has recently gained considerable attention. The biological functions of m7G modification in RNAs, including tRNA, rRNA, mRNA, and miRNA, remain largely unknown in human diseases. Owing to rapid advances in high-throughput technologies, increasing evidence suggests that m7G modification plays a critical role in cancer initiation and progression. As m7G modification and hallmarks of cancer are inextricably linked together, targeting m7G regulators may provide new possibilities for future cancer diagnoses and potential intervention targets. This review summarizes various detection methods for m7G modification, recent advances in m7G modification and tumor biology regarding their interplay and regulatory mechanisms. We conclude with an outlook on the future of diagnosing and treating m7G-related diseases.Copyright © 2023 Elsevier B.V. All rights reserved.