在这项工作中，我们展示了一种基于电化学免疫传感器的新型肿瘤抗原125（CA125）生物标志物检测方法。利用ITO基板作为电极平台，采用导电复合材料碳墨水/碳点/氧化锌（C-ink/CD/ZnO）上的生物标志物作为支持催化性能的抗体（Ab）增强与ZnO的相互作用，作为标签信号分子。它们是化学稳定性、强生物相容性、高导电信号和准确性生物传感器上科学家的关注点。此外，银@聚吡咯（Ag@PPy）的纳米复合材料被用作潜在的氧化还原中介物。与自由标记相比，使用Ag@PPy标记的建筑更加准确。所创建的免疫传感器具有1 ag·mL-1 至100 ng·mL-1的宽线性范围和0.1 fg·mL-1的低检测限，这表明免疫传感器可以被认为是临床中实际样本分析中CA125和其他生物标志物检测的准确和高效诊断工具。版权所有©2023 Elsevier B.V.。保留所有权利。

In this work, we demonstrated a novel cancer antigen 125 (CA125) biomarker detection based on electrochemical immunosensor. The biomarker on conductive composite materials of carbon ink/carbon dot/zine oxide (C-ink/CD/ZnO) was employed as an electrode platform by using ITO substrate to enhance the interaction of antibodies (Ab) with supporting catalytic performance of ZnO as a labeling signal molecule. They were a scientist attention for biosensor with chemical stability, strong biocompatibility, high conductive signal, and accuracy. Moreover, the nanocomposite of silver@polypyrrole (Ag@PPy) was used as a potential redox mediator. The labeled construction with Ag@PPy was more accuracy than that of a free-labeled. The created immunosensor was a wide linear range as 1 ag·mL-1 - 100 ng·mL-1 and a low limitation of detection as 0.1 fg·mL-1 under the optimal condition. This suggested that the immunosensor is considered to be an accurate and efficient diagnostic tool for CA125 and other biomarkers detection in actual sample analysis for clinic.Copyright © 2023 Elsevier B.V. All rights reserved.