乳腺癌是妇女最常见的恶性肿瘤，严重威胁患者的生命和健康。因此，易于敏感地检测人类乳腺癌细胞对癌症诊断至关重要。在这项工作中，利用水热法合成了梅花形CdS/Bi2S3异质结构（CdS/Bi2S3 HSs），并对其光电化学（PEC）特性和生物相容性进行了详细的研究。同时，基于表皮生长因子受体（EGFR）在乳腺癌细胞MDA-MB-231膜上过表达的免疫识别，设计了一种信号放大策略。将上述策略与CdS/Bi2S3 HSs相结合，开发了一种高灵敏度的PEC细胞传感器，用于分析目标MDA-MB-231细胞，展示了1×102∼3×105个细胞/mL更广的线性范围，检测限（LOD）为6个细胞/mL（S/N = 3） 。此外，通过监测药物刺激时表达的EGFR的PEC信号的变化，探讨了该生物传感器用于抗癌药物（例如达可替尼）筛选的潜力。所获得的CdS/Bi2S3 HSs是一种有前途且可行的光活性材料，可用于临床和相关研究中癌细胞的测定和药物筛选。版权©2023 Elsevier B.V.保留所有权利。

Breast cancer is the most common malignant tumor in women, which seriously threatens the life and health of patients. Therefore, facile and sensitive detection of human breast cancer cells is crucial for cancer diagnosis. In this work, plum-branched CdS/Bi2S3 heterostructures (CdS/Bi2S3 HSs) were synthesized under hydrothermal condition, whose photoelectrochemical (PEC) property and biocompatibility were scrutinously investigated. In parallel, a signal amplification strategy was designed based on immune recognition between epidermal growth factor receptor (EGFR) overexpressed on membrane of breast cancer cells MDA-MB-231 and its aptamer. Integration of the above together, a highly sensitive PEC cytosensor was developed for analysis of target MDA-MB-231 cells, exhibiting a wider linear range of 1 × 102 ∼ 3 × 105 cells mL-1 with a limit of detection (LOD) down to 6 cells mL-1 (S/N = 3). Further, the biosensor was explored for anticancer drug (e.g., dacomitinib) screening by monitoring the variations in the PEC signals of the expressed EGFR upon drug stimulation. The obtained CdS/Bi2S3 HSs are identified as promising and feasible photoactive material for determination of cancer cells and drug screening in clinic and related research.Copyright © 2023 Elsevier B.V. All rights reserved.