miR-22-3p通过靶向各种下游基因发挥肿瘤抑制作用，但其在胃癌（GC）中的作用和下游靶点尚待确定。我们旨在探讨miR-22-3p在胃癌中的作用及其潜在机制。使用miR-22-3p模拟物和抑制剂分别过度表达或沉默miR-22-3p的表达。利用定量实时PCR（RT-qPCR）和Western blot分别分析mRNA或蛋白质水平的丰度。实施CCK-8测定、细胞集落形成测定和流式细胞术研究miR-22-3p对胃癌细胞增殖和凋亡的影响。使用荧光素酶分析评估miR-22-3p对糖酵解酶磷酸烯醇酶1（ENO1）表达的作用。在这项研究中，我们发现miR-22-3p在GC细胞中被下调。通过转染miR-22-3p抑制剂或模拟物，我们显示miR-22-3p抑制了GC细胞的增殖和迁移，并引发细胞死亡。此外，我们发现miR-22-3p通过控制乳酸的生成和葡萄糖的消耗参与了糖酵解。TargetScan数据库显示ENO1可能是miR-22-3p的靶标，荧光素酶实验验证了这一假设。恢复实验表明，miR-22-3p抑制的GC细胞的增殖和迁移可以通过过度表达ENO1来恢复。综上所述，我们确定了miR-22-3p / ENO1的新轴心，可作为胃癌发展的治疗靶点。

miR-22-3p functions as a tumor suppressor by targeting a variety of downstream genes, while its role and downstream targets in gastric cancer (GC) remain to be determined. We aimed to explore the role of miR-22-3p in gastric cancer and the potential mechanism.miR-22-3p mimic and inhibitor were used to overexpress or knockdown the expression of miR-22-3p separately. Quantitative real-time PCR (RT-qPCR) and Western blot were used to analyse the abundance of mRNA or protein level respectively. CCK-8 assay, cell colony formation assay, and flow cytometry were implemented to investigate the effect of miR-22-3p on gastric cancer cell proliferation and apoptosis. Luciferase assay was used to evaluate the role of miR-22-3p on the expression of glycolytic enzyme enolase 1 (ENO1).In this study, we found that miR-22-3p was downregulated in GC cells. By transfecting the cells with miR-22-3p inhibitors or mimics, we showed that miR-22-3p suppressed GC cell proliferation and migration, as well as triggered cell death. In addition, we discovered that miR-22-3p was engaged in glycolysis by controlling the generation of lactate as well as the consumption of glucose. TargetScan database suggested that the ENO1 may be a target of the miR-22-3p, and the luciferase experiment verified this hypothesis. Recovery assays showed that the proliferation and migration of GC cells suppressed by miR-22-3p could be rescued by overexpression of ENO1.Collectively, we identified a new axis of miR-22-3p/ENO1 for GC development, which could be investigated as a therapeutic target for GC.