一氧化氮（NO）在多种生理功能中起着至关重要的作用，因此其实时感应非常重要。本研究开发了一种集成纳米电子系统，包括钴单原子纳米酶（Co-SAE）芯片阵列传感器和电子信号处理模块（INDCo-SAE），用于正常和肿瘤小鼠体内外的NO多通道定量。由于Co-SAE具有高原子利用率和催化活性，因此其NO的线性范围可达36至4.1×105 nM，检测限低至12 nM。结合原位衰减全反射表面增强红外光谱（ATR-SEIRAS）测量和密度泛函计算揭示了Co-SAE对NO的激活机制。各器官产生NO的行为得到了研究。研究结果表明，使用设计良好的设备对受伤小鼠所产生的NO产量进行评估，发现其约为正常小鼠的15倍。本研究填补了体外和体内分子分析生物传感器和集成系统之间的技术差距。所制备的集成无线纳米电子系统具有多个测试通道，这显着提高了检测效率，并可广泛用于设计具有多重分析能力的便携式传感器。

Nitric oxide (NO) exhibits a crucial role in various versatile and distinct physiological functions. Hence, its real-time sensing is highly important. Herein, we developed an integrated nanoelectronic system comprising a cobalt single-atom nanozyme (Co-SAE) chip array sensor and an electronic signal processing module (INDCo-SAE) for both in vitro and in vivo multichannel qualifying of NO in normal and tumor-bearing mice. The high atomic utilization and catalytic activity of Co-SAE endowed an ultrawide linear range for NO varying from 36 to 4.1 × 105 nM with a low detection limit of 12 nM. Combining in situ attenuated total reflectance surface enhanced infrared spectroscopy (ATR-SEIRAS) measurements and density function calculation revealed the activating mechanism of Co-SAE toward NO. The NO adsorption on an active Co atom forms *NO, followed by the reaction between *NO and OH-, which could help design relevant nanozymes. Further, we investigated the NO-producing behaviors of various organs of both normal and tumor-bearing mice using the proposed device. We also evaluated the NO yield produced by the wounded mouse using the designed device and found it to be approximately 15 times that of the normal mouse. This study bridges the technical gap between a biosensor and an integrated system for molecular analysis in vitro and in vivo. The as-fabricated integrated wireless nanoelectronic system with multiple test channels significantly improved the detection efficiency, which can be widely used in designing other portable sensing devices with multiplexed analysis capability.