口腔白斑伴口腔粘膜下纤维化是一种高风险口腔潜在恶性疾病，但其免疫微环境知之甚少。从两家医院收集了30个口腔白斑伴口腔粘膜下纤维化、30个口腔白斑和30个口腔粘膜下纤维化样本。进行免疫组织化学分析T细胞标志物（CD3、CD4、CD8和Forkhead box P3（Foxp3））的表达情况、B细胞标志物（CD20）、巨噬细胞标志物（CD68和CD163）、免疫抑制受体配体（PD-L1）和Ki-67。口腔白斑伴口腔粘膜下纤维化中CD3+（p＜0.001）、CD4+（p＝0.018）和CD8+（p＝0.031）细胞的数量少于口腔白斑的数量。口腔白斑伴口腔白斑的CD4+细胞数量（p＝0.035）高于口腔粘膜下纤维化。口腔白斑中CD3+（p＜0.001），CD4+（p＜0.001），Foxp3+（p＝0.019）和CD163+（p＝0.029）细胞的数量均比口腔粘膜下纤维化多。口腔白斑伴口腔粘膜下纤维化、口腔白斑和口腔粘膜下纤维化中观察到不同程度的免疫浸润。免疫微环境的特征化有助于个性化免疫治疗。© 2023 John Wiley＆Sons A/S。由John Wiley＆Sons Ltd.发表。

Oral leukoplakia concomitant with oral submucous fibrosis is a high-risk oral potentially malignant disorder, but little is known about its immune microenvironment.Thirty samples of oral leukoplakia concomitant with oral submucous fibrosis, 30 oral leukoplakia samples, and 30 oral submucous fibrosis samples were collected from two hospitals. Immunohistochemistry was performed to analyze expression of T cell biomarkers [CD3, CD4, CD8, and Forkhead box P3 (Foxp3)], a B cell biomarker (CD20), macrophage biomarkers (CD68 and CD163), an immune inhibitory receptor ligand (PD-L1), and Ki-67.The numbers of CD3+ (p < 0.001), CD4+ (p = 0.018), and CD8+ (p = 0.031) cells in oral leukoplakia concomitant with oral submucous fibrosis were less than those in oral leukoplakia. The number of CD4+ cells (p = 0.035) in oral leukoplakia concomitant with oral leukoplakia was higher than that in oral submucous fibrosis. More CD3+ (p < 0.001), CD4+ (p < 0.001), Foxp3+ (p = 0.019), and CD163+ (p = 0.029) cells were found in oral leukoplakia than in oral submucous fibrosis.Various levels of immune infiltration were observed among oral leukoplakia concomitant with oral submucous fibrosis, oral leukoplakia, and oral submucous fibrosis. Characterization of the immune microenvironment may contribute to personalized immunotherapy.© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.