为了确定B细胞淋巴瘤6（BCL6）在着床窗口期内的差异表达情况，与常用于冷冻胚胎移植的各种子宫准备方案相关的子宫内膜异位症标志物被推测可用于预测孕产不良结果。回顾性队列研究；地点：学术中心；受试者：进行子宫内膜活组织检查以评估BCL6的不孕症患者；接受的医学处理：外源性雌二醇和/或孕酮；主要结局指标：子宫内膜BCL6 HSCORE 和过度表达（HSCORE > 1.4）；结果：分析了244名患者：76名患者在自然月经周期中未接受外源性激素曝露（NC），25名患者在改良的自然周期胚胎移植方案下进行了取样，其方案包括注射绒毛膜促性腺激素α和黄体期阴道孕酮补充（mNC），143名患者在计划周期胚胎移植方案下进行了取样，其方案涉及雌二醇和肌肉内注射孕酮油（PC）。NC 中的中位数 HSCORE（四分位数范围）最高 [3.0（1.8-3.6）]。相比于 NC，mNC [1.1（0.4-2.1）]（p<0.01）及 PC 组 [0.8（0.3-1.3）]（p<0.01）的 BCL6 表达显著较低。BCL6 过度表达（HSCORE >1.4）在 NC 中观察到 80.3％，在 mNC 中观察到 40.0％，而在 PC 中观察到 23.1％（p<0.01）。调整协变量后，接受外源性孕酮处理的子宫内膜，与自然月经周期相比，BCL6 过度表达的几率明显降低（mNC 的调整 OR 0.12 [95％ CI 0.04-0.35]，p<0.01，PC 的 OR 0.08 [95％CI 0.04-0.17]，p<0.01）。BCL6 的表达因子宫准备方法的不同而不同，在使用外源性孕酮情况下，表达水平较低。医疗性子宫内膜状态下 BCL6 测试的可靠性和实用性需要进一步研究。版权所有©2023。Elsevier出版。

To determine if B-cell lymphoma 6 (BCL6), an endometriosis-associated marker postulated to predict poor pregnancy outcomes, is differentially expressed in the window of implantation with various uterine preparation regimens commonly used for frozen embryo transfers.Retrospective cohort study SETTING: Academic center SUBJECTS: Patients with infertility who underwent endometrial biopsy for BCL6 evaluation EXPOSURE: Exogenous estradiol and/or progesterone MAIN OUTCOME MEASURES: Endometrial BCL6 HSCORE and overexpression (HSCORE >1.4) RESULTS: Two hundred and forty-four patients were included in the analysis: 76 patients were sampled in a natural menstrual cycle without exogenous hormone exposure (NC), 25 under a modified natural cycle embryo transfer protocol with choriogonadotropin alfa injection followed by luteal phase vaginal progesterone supplementation (mNC), and 143 under a programmed cycle embryo transfer protocol, with estradiol administration followed by addition of intramuscular progesterone-in-oil injections (PC). Median HSCORE (interquartile range) was the highest in NC [3.0 (1.8-3.6)]. BCL6 expression was significantly lower in mNC [1.1 (0.4-2.1)] (p<0.01), and PC groups [0.8 (0.3-1.3)] (p<0.01) compared to NC. BCL6 overexpression (HSCORE >1.4) was observed in 80.3% of NC, 40.0 % of mNC, and 23.1 % of PC (p<0.01). After adjusting for covariates, endometrium exposed to exogenous progesterone had significantly lower odds of BCL6 overexpression compared to that of a natural menstrual cycle (adjusted OR 0.12 [95% CI 0.04-0.35], p<0.01 for mNC, and OR 0.08 [95%. CI 0.04-0.17], p<0.01 for PC).BCL6 expression differs by type of uterine preparation method, with lower levels observed with exogenous progesterone exposure. The validity and utility of BCL6 testing under medicated endometrial states warrants further investigation.Copyright © 2023. Published by Elsevier Inc.