检测生长因子结合蛋白2相关结合蛋白2(Gab2)和CT10调节酶II(CrkII)在卵巢癌中的表达以及分析其临床意义。探讨Gab2和CrkII对卵巢癌细胞生物学行为的影响。分析Gab2在卵巢癌发展中的可能分子机制。利用免疫组化检测卵巢癌中Gab2和CrkII的表达情况。利用卡方检验分析Gab2、CrkII和临床参数之间的关系。利用Cox回归模型评估影响预后的危险因素。通过Kaplan-Meier分析Gab2、CrkII与生存率之间的关系。进行细胞实验，探索Gab2和CrkII对细胞生物学行为的影响。利用免疫共沉淀探究Gab2和CrkII之间的相互作用。免疫组化显示Gab2和CrkII在卵巢癌中表达高。高表达Gab2或CrkII的患者其国际妇产科联盟(FIGO)分期、分级和铂抗药性复发均较高。多元分析显示Gab2和CrkII是独立的预后因子。Kaplan-Meier曲线表明，Gab2和CrkII越高，患者预后越差。观察到Gab2和CrkII的过表达促进了细胞的增殖、转移并降低了化疗敏感性。相反，Gab2和CrkII的敲降则有相反的结果。在CrkII敲降细胞中，我们发现Gab2通过CrkII介导生物学行为。在卵巢癌中，Gab2和CrkII表达增加。Gab2和CrkII的高表达预示患者预后差。Gab2和CrkII促进细胞增殖和迁移，降低化疗敏感性。Gab2通过CrkII调控卵巢癌细胞的生物学行为。© 2023. The Author(s)。

To detect the expression of Growth factor binding protein 2 associated binding protein 2 (Gab2) and CT10 regulator of kinase II (CrkII) in ovarian cancer and analyze their clinical significance. To explore the effects of Gab2 and CrkII on the biological behavior of ovarian cancer cells. To analyze the possible molecular mechanism of Gab2 in the development of ovarian cancer.Immunohistochemistry was used to detect the expression of Gab2 and CrkII in ovarian cancer. Chi square test was used to analyze the correlation between Gab2, CrkII and clinical parameters. Using Cox regression model to evaluate the risk factors affecting the prognosis. To analyze the correlation between Gab2, CrkII and survival rate by Kaplan-Meier. Cell experiments were preformed to explore the effects of Gab2 and CrkII on the biological behavior of cells. The interaction between Gab2 and CrkII was explored by immunoprecipitation.Immunohistochemistry revealed that high expression of Gab2 and CrkII in ovarian cancer. Patients with high expression of Gab2 or CrkII had higher International Federation of Gynecology and Obstetrics (FIGO) stage, grade and platinum-resistance recurrence. Multivariate analysis showed that Gab2 and CrkII were independent prognostic factors. Kaplan-Meier curve showed that the higher Gab2 and CrkII were, the poor prognosis the patients had. We observed that the overexpression of Gab2 and CrkII promoted the proliferation, metastasis and reduced chemosensitivity of cells. Conversely, the knockdown of Gab2 and CrkII resulted in the opposite results. In CrkII-knockdown cells, we found that Gab2 mediates biological behavior through CrkII.The expression of Gab2 and CrkII increase in ovarian cancer. The higher expression of Gab2 and CrkII predict the poor prognosis of patients. Gab2 and CrkII promote the proliferation and migration and reduce the chemosensitivity of cells. Gab2 regulates the biological behaviors of ovarian cancer cells through CrkII.© 2023. The Author(s).