尽管进行了相当多的预防和治疗研究，肺癌仍然是一个重大的健康问题。通过更深入地了解肿瘤，可以使用特异性高的患者特异性体外球模型来准确地研究肺癌的原因、转移和治疗策略。本文介绍了生物制造的肺肿瘤模型，包括患者来源的肿瘤球体、内皮细胞和肺脱细胞外基质，能够保持放射状氧梯度以及肿瘤的生物物理化学行为，用于精密医学。同时表明，开发的肺癌球体模型在联合临床试验中能够以85%的准确率、86.7%的敏感性和80%的特异性重现患者对化疗和靶向治疗的反应。RNA测序分析验证了球体中的基因表达与患者原发性肿瘤中的基因表达相似。该模型可作为一种体外预测模型，用于个性化癌症治疗和提高临床护理质量。© 2023 Wiley-VCH GmbH发表的《Advanced Science》。

Lung cancer remains a major health problem despite the considerable research into prevention and treatment methods. Through a deeper understanding of tumors, patient-specific ex vivo spheroid models with high specificity can be used to accurately investigate the cause, metastasis, and treatment strategies for lung cancer. Biofabricate lung tumors are presented, consisting of patient-derived tumor spheroids, endothelial cells, and lung decellularized extracellular matrix, which maintain a radial oxygen gradient, as well as biophysicochemical behaviors of the native tumors for precision medicine. It is also demonstrated that the developed lung-cancer spheroid model reproduces patient responses to chemotherapeutics and targeted therapy in a co-clinical trial, with 85% accuracy, 86.7% sensitivity, and 80% specificity. RNA sequencing analysis validates that the gene expression in the spheroids replicates that in the patient's primary tumor. This model can be used as an ex vivo predictive model for personalized cancer therapy and to improve the quality of clinical care.© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.