本研究的目的是探究硝叶菜碱（NC）和蟾酥酒精提取物（BF）对造血肿瘤细胞程序性细胞死亡（PCD）的影响。使用不同剂量的NC和BF暴露于造血肿瘤细胞，以测量生长抑制水平。同时，利用倒置显微镜观察细胞形态，利用Western blot技术检测与凋亡相关的蛋白质表达水平。NC和BF对造血肿瘤细胞的影响不同。虽然在倒置显微镜下可以看到异常的细胞形态，但Western blot的结果显示这两种药物通过不同的途径诱导PCD。药物抵抗性的强度在不同的细胞中有所变化。THP-1、Jurkat和RPMI-8226在BF中的半数抑制浓度（IC50）分别为36.23 nM、26.71 nM和40.46 nM，在NC中分别为9.24 µM、4.33 µM和28.18 µM。不同的造血恶性细胞表现出不同程度的药物抵抗性，NC和BF引发造血肿瘤细胞凋亡的机制也各不相同。© 2023. The Author(s).

The objective of this work to study the programmed cell death (PCD) in hematological tumor cells induced by nitidine chloride (NC) and bufalin (BF). Hematological tumor cells were exposed to various doses of NC and BF to measure the level of growth inhibition. While inverted microscope is used to observe cell morphology, western blot technique is used to detect apoptosis-related protein expression levels. The effects of NC and BF on hematological tumor cells were different. Although abnormal cell morphology could be seen under the inverted microscope, the western blot results showed that the two medicines induced PCD through different pathways. Drug resistance varied in intensity across distinct cells. THP-1, Jurkat, and RPMI-8226 each had half maximum inhibitory concentrations (IC50) of 36.23 nM, 26.71 nM, and 40.46 nM in BF, and 9.24 µM, 4.33 µM, and 28.18 µM in NC, respectively. Different hematopoietic malignancy cells exhibit varying degrees of drug resistance, and the mechanisms by which apoptosis of hematologic tumor cells is triggered by NC and BF are also distinct.© 2023. The Author(s).