估计肾小球功能对于诊断肾脏疾病是必要的。然而，在临床中，对肾小球的研究目前是通过尿液和血液检测间接进行的。最近出现了一种称为超声定位显微镜（ULM）的成像技术。它基于记录血液中单个微泡的连续运动的能力。尽管ULM将血管成像的分辨率提高了十倍，但最小血管的成像尚未报告。我们从人和大鼠身上获得超声序列，然后应用滤波器将数据集分成缓慢移动和快速移动的微泡。我们进行了双重跟踪来突出和表征具有单一行为的微泡族群，我们决定将这种技术称为“感应ULM”（sULM）。我们在大鼠的尸体中使用末期微CT来进行并排确认。在这项研究中，我们报告了肾小球中微泡流动的观察结果，提出了一套分析工具，可以从单个微泡中提取数量信息，例如剩磁时间或标准化距离。由于肾小球在肾脏功能中起着关键作用，因此观察它们可能会产生更深入的了解。它也可以成为诊断患者肾脏疾病的工具。更普遍地说，它将使成像能力更接近器官的功能单元，这是理解大多数疾病的关键，例如癌症、糖尿病或肾衰竭。本研究由欧洲研究理事会在欧盟地平线H2020计划下资助（ERC Consolidaator grant agreement No 772786-ResolveStroke）。版权所有©2023作者。由Elsevier BV出版，保留所有权利。

Estimation of glomerular function is necessary to diagnose kidney diseases. However, the study of glomeruli in the clinic is currently done indirectly through urine and blood tests. A recent imaging technique called Ultrasound Localization Microscopy (ULM) has appeared. It is based on the ability to record continuous movements of individual microbubbles in the bloodstream. Although ULM improved the resolution of vascular imaging up to tenfold, the imaging of the smallest vessels had yet to be reported.We acquired ultrasound sequences from living humans and rats and then applied filters to divide the data set into slow-moving and fast-moving microbubbles. We performed a double tracking to highlight and characterize populations of microbubbles with singular behaviors. We decided to call this technique "sensing ULM" (sULM). We used post-mortem micro-CT for side-by-side confirmation in rats.In this study, we report the observation of microbubbles flowing in the glomeruli in living humans and rats. We present a set of analysis tools to extract quantitative information from individual microbubbles, such as remanence time or normalized distance.As glomeruli play a key role in kidney function, it would be possible that their observation yields a deeper understanding of the kidney. It could also be a tool to diagnose kidney diseases in patients. More generally, it will bring imaging capabilities closer to the functional units of organs, which is a key to understand most diseases, such as cancer, diabetes, or kidney failures.This study was funded by the European Research Council under the European Union Horizon H2020 program (ERC Consolidator grant agreement No 772786-ResolveStroke).Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.