脓毒症诱导的心肌功能障碍是死亡率和发病率的主要原因。最近的研究表明，炎症和氧化应激在脂多糖（LPS）诱导的心脏损伤的病理生理学中起着中心作用。乳香树脂萃取物被传统民间医学用于治疗各种慢性炎症性疾病已有数个世纪。本研究旨在研究乳香树脂预处理对H9c2细胞中LPS诱导的心脏损伤的影响。细胞经过不同浓度的乳香树脂（5-45μg/ml）预处理24小时后，再用LPS（10μg/ml）刺激24小时。然后，使用酶联免疫吸附实验（ELISA）、实时PCR或适当的生化方法测定细胞存活率、肿瘤坏死因子（TNF）-α、前列腺素（PGE）-2、白细胞介素（IL）-1β、IL-6、诱导型一氧化氮合酶（iNOS）、环氧合酶（COX）-2、一氧化氮（NO）和谷胱甘肽（GSH）的水平。我们的结果表明，LPS处理会显着降低细胞存活率和GSH水平，相反，会显著增加炎症标志物和NO的基因和蛋白质表达水平。然而，乳香树脂的预处理（5、15和45μg/ml）降低了TNF-α、PGE2、IL-1β、COX-2、iNOS、IL-6和NO的产生，同时提高了细胞存活率和GSH水平。综上所述，我们的结果表明，乳香树脂可能是一种潜在的治疗剂，通过其抗炎和抗氧化特性对抗LPS和内毒素血症诱导的心脏损伤。版权所有 © 2023 Elsevier Ltd.

Sepsis-induced myocardial dysfunction is the main reason for mortality and morbidity. Recent investigations have shown that inflammation and oxidative stress play a central role in lipopolysaccharide (LPS)-induced cardiac injury pathophysiology. Gum-resin extracts of Boswellia serrata have been traditionally used in folk medicine for centuries to treat various chronic inflammatory diseases. The present study aimed to investigate the effects of B. serrata pretreatment on LPS-induced cardiac damage in H9c2 cells. The cells were pretreated with various concentrations of B. serrata (5-45 μg/ml) for 24 h and then stimulated with LPS (10 μg/ml) for another 24 h. Afterward, the levels of cell viability, tumor necrosis factor (TNF)-α, prostaglandin (PGE)-2, interleukin (IL)-1β, IL-6, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, nitric oxide (NO) and glutathione (GSH) were determined using enzyme-linked immunosorbent assay (ELISA), real time-PCR or appropriated biochemical methods. Our results demonstrated that LPS treatment caused a remarkable decrease in cell viability and GSH, and on the contrary, it led to a significant increase in the levels of gene and protein expression of inflammatory markers and NO. However, pretreatment of B. serrata (5, 15, and 45 μg/ml) decreased the levels of TNF-α, PGE2, IL-1β, COX-2, iNOS, IL-6, and NO production, while cell viability and GSH levels were increased. Taken together, our results demonstrated that B. serrata might be a potential therapeutic agent against LPS and endotoxemia-induced cardiac injury, through its anti-inflammatory and antioxidant properties.Copyright © 2023. Published by Elsevier Ltd.