本研究的目的在于检查急性髓性白血病（AML）患者骨髓中是否存在细菌组成基因组。我们重新分析了7例AML患者骨髓样本的全基因组测序数据，其中4例在治疗后缓解，用于进行宏基因组学分析。在去除人类序列后，利用无法映射的序列对物种层面组成进行了描述。我们使用宏基因组分离方法来确认识别到的生物种是已知或新品系的完整基因组。我们观察到了一种独特的新型微生物特征，其中在5例患有AML或缓解的患者中，Carnobacterium maltaromaticum是最常见的物种。在所有样本中均发现C. maltaromaticum“BMAML_KR01”的完整基因组，完整性为100％，杂质率为8.5％，基于单核苷酸多态性变异，它与C. maltaromaticum品系DSM20730和SF668紧密聚类。我们在BMAML_KR01基因组中鉴定了五种可促进癌症进展的独特蛋白质和104种致病因子蛋白质。据我们所知，这是首次报告AML患者中C. maltaromaticum新品系的发现。C. maltaromaticum及其新品系的存在表明有必要验证该细菌的存在并评估其病理生理作用。 © 2023. 作者，在独家许可下授权给 Springer Nature Switzerland AG。

The aim of this study aimed to examine the existence of a bacterial metagenome in the bone marrow of patients with acute myeloid leukemia (AML). We re-examined whole-genome sequencing data from the bone marrow samples of seven patients with AML, four of whom were remitted after treatment, for metagenomic analysis. After the removal of human reads, unmapped reads were used to profile the species-level composition. We used the metagenomic binning approach to confirm whether the identified taxon was a complete genome of known or novel strains. We observed a unique and novel microbial signature in which Carnobacterium maltaromaticum was the most abundant species in five patients with AML or remission. The complete genome of C. maltaromaticum "BMAML_KR01," which was observed in all samples, was 100% complete with 8.5% contamination and closely clustered with C. maltaromaticum strains DSM20730 and SF668 based on single nucleotide polymorphism variations. We identified five unique proteins that could contribute to cancer progression and 104 virulent factor proteins in the BMAML_KR01 genome. To our knowledge, this is the first report of a new strain of C. maltaromaticum in patients with AML. The presence of C. maltaromaticum and its new strain in patients indicates an urgent need to validate the existence of this bacterium and evaluate its pathophysiological role.© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.