通过三维组织的细胞浸润/迁移不仅对生理/病理过程至关重要，而且是癌症恶性的标志。然而，如何量化三维细胞迁移/浸润的时空动态是有挑战性的。本文报道了一种基于电机耦合芯片系统的三维细胞侵袭/迁移测定法（3D-CIMA），能够以实时、无标记、非破坏性和高通量的方式监测三维细胞浸润/迁移的时空动态。结合三维拓扑网络和复杂阻抗检测技术，该研究表明，3D-CIMA可定量表征具有可控生物物理/生物力学特性的三维细胞外基质（ECM）中癌细胞的集体浸润/迁移动力学。更重要的是，该研究进一步揭示了它不仅具有在三维微环境中进行抗肿瘤药物定量评估并减少细胞培养尺寸影响的能力，还可以分级临床癌症标本。所提出的3D-CIMA为研究细胞与三维细胞外微环境的相互作用提供了一种新的定量方法，具有在机械生物学、药物筛选甚至精准医学等各领域的潜在应用。© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.

Cell invasion/migration through three-dimensional (3D) tissues is not only essential for physiological/pathological processes, but a hallmark of cancer malignancy. However, how to quantify spatiotemporal dynamics of 3D cell migration/invasion is challenging. Here, this work reports a 3D cell invasion/migration assay (3D-CIMA) based on electromechanical coupling chip systems, which can monitor spatiotemporal dynamics of 3D cell invasion/migration in a real-time, label-free, nondestructive, and high-throughput way. In combination with 3D topological networks and complex impedance detection technology, this work shows that 3D-CIMA can quantitively characterize collective invasion/migration dynamics of cancer cells in 3D extracellular matrix (ECM) with controllable biophysical/biomechanical properties. More importantly, this work further reveals that it has the capability to not only carry out quantitative evaluation of anti-tumor drugs in 3D microenvironments that minimize the impact of cell culture dimensions, but also grade clinical cancer specimens. The proposed 3D-CIMA offers a new quantitative methodology for investigating cell interactions with 3D extracellular microenvironments, which has potential applications in various fields like mechanobiology, drug screening, and even precision medicine.© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.