藻蓝蛋白提供有利的生物效应，包括免疫调节、抗癌、抗氧化和抗炎能力。虽然作为天然色素分子，藻蓝蛋白与合成色素不同，但在高温和光照条件下易降解。本研究仔细研究了超声处理对PC和海带多酚复合物的结构和功能特性的影响。采用紫外-可见光谱、荧光光谱、循环二色性（CD）光谱和傅里叶红外光谱（FTIR）研究了超声处理后PC和海带多酚之间的相互作用。此外，还评估了复合物的抗氧化潜力和体外消化性。结果表明，PC的UV-Vis光谱出现了降解效应、荧光淬灭效应和CD光谱的弱构象变化。X射线衍射（XRD）数据表明，PC被鉴定为非晶态，而海带多酚被嵌入到PC基质中。差示扫描量热法（DSC）结果显示，超声处理和加入海带多酚可以提高PC的变性峰温度（Td）至78.7°C。体外消化和自由基清除实验表明，适当的超声处理和海带多酚加入更能耐受模拟肠道条件，并能改善DPPH和ABTS+自由基清除性能。超声处理和海带多酚的加入改变了PC的结构和功能特性。这些结果证明了通过超声辅助从海带中提取多酚类可改善PC的功能特性的可行性，并提供了将PC-多酚类复合物作为功能性食品成分或生物活性材料的可能性。版权所有©2023 Bai，Li，Xie，Wang，Dong和Qi。

Phycocyanin offers advantageous biological effects, including immune-regulatory, anticancer, antioxidant, and anti-inflammation capabilities. While PC, as a natural pigment molecule, is different from synthetic pigment, it can be easily degradable under high temperature and light conditions.In this work, the impact of ultrasound treatment on the complex of PC and phlorotannin structural and functional characteristics was carefully investigated. The interaction between PC and phlorotannin after ultrasound treatment was studied by UV-Vis, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, fourier transform infrared (FTIR) spectroscopy. Additionally, the antioxidant potential and in vitro digestibility of the complexes were assessed.The result was manifested as the UV-Vis spectrum reduction effect, fluorescence quenching effect and weak conformational change of the CD spectrum of PC. PC was identified as amorphous based on the X-ray diffraction (XRD) data and that phlorotannin was embedded into the PC matrix. The differential scanning calorimetry (DSC) results showed that ultrasound treatment and the addition of phlorotannin could improve the denaturation peak temperatures (Td) of PC to 78.7°C. In vitro digestion and free radical scavenging experiments showed that appropriate ultrasound treatment and the addition of phlorotannin were more resistant to simulated gastrointestinal conditions and could improve DPPH and ABTS+ free radical scavenging performance.Ultrasound treatment and the addition of phlorotannin changed the structural and functional properties of PC. These results demonstrated the feasibility of ultrasound-assisted phlorotannin from A. nodosum in improving the functional properties of PC and provided a possibility for the application of PC-polyphenol complexes as functional food ingredients or as bioactive materials.Copyright © 2023 Bai, Li, Xie, Wang, Dong and Qi.