腺癌不另分类（AC）和黏液腺癌（MC）具有不同的生物学行为和临床特征。我们利用之前的蛋白质组数据和公共转录组、单细胞转录组和空间转录组数据库，对MC、AC和正常结肠组织的肿瘤微环境进行了分子图谱分析。通过探索AC和MC的一般和特定分子特征，我们发现AC是免疫活性的，但暴露在缺氧微环境中。MC细胞可以保护DNA免受损伤，而微环境不利于白细胞經内皮细胞迁移。我们为未来研究确定了AC和MC的几个潜在分子和细胞靶点。我们还强调，AC和MC之间的主要区别可能不在于细胞类型和功能的多样性，而可能在于细胞相互作用。基质和上皮细胞的相互作用在MC和AC中发挥着重要作用，但涉及不同的调节途径。版权所有 © 2023 Xu，Zheng，Li，Shao和Yin。

Adenocarcinoma not otherwise specified (AC) and mucinous adenocarcinoma (MC) have different biological behaviors and clinical features. We utilized our previous proteomic data and public transcriptome, single-cell transcriptome, and spatial transcriptome databases to profile the molecular atlas of the tumor microenvironments of MC, AC, and normal colon tissues. By exploring the general and specific molecular features of AC and MC, we found that AC was immune-active but exposed to a hypoxic microenvironment. MC cells could protect against DNA damage, and the microenvironment was unfavorable to leukocyte transendothelial migration. We identified several potential molecular and cellular targets of AC and MC for future research. We also highlighted that the major difference between AC and MC was not the variety of cell types and functions but possibly cell interactions. Stromal and epithelial cell interactions play important roles in both MC and AC, but different regulatory pathways were involved.Copyright © 2023 Xu, Zheng, Li, Shao and Yin.