miR-219-1基因的各种变体是文献中最早与非小细胞肺癌预后相关的基因之一。我们的目的是对miR-219-1基因的两种不同变体进行基因型分型，并研究其作为生物标志物在NSCLC的诊断和治疗中的应用。根据International NSCLC标准选择患者，并从血液（138名患者和100名健康个体）中分离出基因组DNA。然后应用qRT-PCR确定miR-219-1基因多态性的rs213210和rs421446变体。使用Pearson卡方检验和Fisher确切检验比较等位基因和基因型频率。我们发现，rs213210中的TT基因型（p=0.381）与CC基因型（p=0.165）以及rs421446中的CC基因型（p=0.823）与TT基因型（p=0.537）相比并没有显示出显著增加的NSCLC风险。miR-219-1的多态性与NSCLC的结果之间没有关系。miRNA单核苷酸多态性可用作预测癌症易感性、早期诊断和预后的遗传标记。我们的研究表明，miR-219-1的两种变体与土耳其人群中的NSCLC无关。这可能是由于种族、地区和人口背景的差异造成的，这些差异可能导致结果各异。

Various variants of the miR-219-1 gene are one of the first genes associated with NSCLC prognosis in the literature.We aimed to genotype two different variants of the miR-219-1 gene and to investigate to using of the result as a biomarker in the diagnosis and treatment of NSCLC.The patients were chosen according to International NSCLC criteria and genomic DNA was isolated from blood (138 patients and 100 healthy individuals). Then qRT-PCR was applied to determine the rs213210 and rs421446 variants of miR-219-1 gene polymorphisms. Allele and genotype frequencies were compared using Pearson's chi-square and Fisher's exact tests test.We found that TT genotype (p=0,381) in rs213210 compared with CC genotype (p=0,165) and CC genotype (p=0,823) in rs421446 compared with TT genotype (p=0,537) did not show a significantly increased risk of NSCLC. There is no relationship between polymorphisms in miR-219-1 and the outcome of NSCLC.miRNA single nucleotide polymorphisms can be used as genetic biomarkers to predict cancer susceptibility, early diagnosis, and prognosis. Our study has shown that two variants of miR-219-1 were not related to NSCLC in the Turkish population. The reason for this can be differences in ethnicity, regions, and background of population and these differences could lead to various outcomes.© 2022 Tas SK et al.