基于核酸的分析生物平台已经成为基因组学诊断测试和癌症等疾病早期检测工具的重要手段。在这种情况下，我们报告了一种安培计生物平台的开发，用于测定特定的人乳头状瘤病毒16型（HPV16）序列。该生物平台采用免疫核酸杂交夹心法。一种与选定的HPV16目标DNA序列互补的生物素化RNA捕获探针（RNAbCp）被固定在链霉亲和素包被磁微珠（Strep-MBs）的表面上。RNAbCP和HPV16目标序列杂交形成的RNA/DNA杂交物被一种商业抗体识别，该抗体专门结合到杂交物（AbDNA-RNA）。辣根过氧化物酶标记的二抗（antiIgG-HRP）用于检测AbDNA-RNA。在H2O2和氢醌（HQ）存在的情况下，依靠检测屏幕印刷电极（SPCEs）上捕获的标记有HRP的磁性生物结合物的安培检测，该生物工具的低检测限（0.5pM）可用于合成HPV16目标DNA。此外，该开发的生物平台能够在45分钟的测试时间内仅使用25 ng的扩增DNA区别于HPV16阳性和阴性的人类癌细胞。版权所有©2023 Elsevier B.V.。

Nucleic acid-based analytical bioplatforms have gained importance as diagnostic tests for genomics and as early detection tools for diseases such as cancer. In this context, we report the development of an amperometric bioplatform for the determination of a specific human papillomavirus type 16 (HPV16) sequence. The bioplatform utilizes an immune-nucleic acid hybrid-sandwich assay. A biotinylated RNA capture probe (RNAbCp), complementary to the selected HPV16 target DNA sequence, was immobilised on the surface of streptavidin coated magnetic microbeads (Strep-MBs). The RNA/DNA heteroduplex resulting from the hybridization of the RNAbCP and the HPV16 target sequence was recognised by a commercial antibody that specifically bound to the heteroduplex (AbDNA-RNA). A horseradish-peroxide labeled secondary antibody (antiIgG-HRP) was used for the detection of AbDNA-RNA. Relying on amperometric detection of the resulting HRP-labeled magnetic bioconjugates captured on screen-printed electrodes (SPCEs) in the presence of H2O2 and hydroquinone (HQ), the biotool achieved a low limit of detection (0.5 pM) for the synthetic HPV16 target DNA. In addition, the developed bioplatform was able to discriminate between HPV16 positive and negative human cancer cells using only 25 ng of amplified DNA in a test time of 45 min.Copyright © 2023 Elsevier B.V. All rights reserved.