研究集中超声波消融手术（FUAS）治疗多个纤维腺瘤（FA）的组织病理学发现和随访结果。共纳入20名患者101个多发性FAs。FUAS消融一次后，有21个≥15.0mm的损伤在一周内手术切除进行病理组织学分析，包括2,3,5-三苯基四唑盐（TTC）染色，H&E染色，烟酰胺腺嘌呤二核苷酸（NADH）-黄酮酶染色，透射电子显微镜（TEM）和扫描电子显微镜（SEM）。其余80个病变在治疗后3、6和12个月进行随访。所有消融手术均成功完成。病理学结果表明FA的不可逆损伤已得到证实。TTC、H&E和NADH染色以及TEM / SEM分别证明肿瘤细胞死亡和肿瘤结构在毛、细胞和亚细胞水平上被破坏。 FUAS治疗后12个月的中位缩小率为66.4（43.6，89.5）％。FUAS治疗后的纤维腺瘤的病理学分析证明，FUAS可以有效地诱导FA不可逆性凝固性坏死，并且瘤体容积将随着随访逐渐缩小。 FUAS是治疗多个FA并获得良好产生的安全而有效的方法。 要点 这项研究是FUAS治疗后FA详细的组织病理学分析的第一项研究。 FUAS可以有效地诱导纤维腺瘤细胞的不可逆凝固性坏死。 FUAS消融多个纤维腺瘤是安全而有效的。

To investigate the histopathological findings and follow-up outcome of focused ultrasound ablation surgery (FUAS) treatment of multiple fibroadenomas (FA).A total of 20 patients with 101 multiple FAs were enrolled. After one session FUAS ablation, 21 lesions (≥15.0 mm) were surgically removed within one week for histopathological analysis, including 2, 3, 5-triphenyltetrazolium chloride (TTC) staining, H&E staining, nicotinamide adenine dinucleotide (NADH) -flavretin enzyme staining, Transmission electron microscope (TEM) and scanning electron microscope (SEM). The remaining 80 lesions were followed up at 3, 6 and 12 months after treatment.All ablation procedures were performed successfully. Pathologic findings showed that irreversible damage of FA was confirmed. TTC, H&E and NADH staining and TEM/SEM demonstrated tumor cell death and tumor structural destruction at the gross, cellular, and subcellular levels, respectively. The median shrinkage rate at 12 months post-FUAS was 66.4 (43.6, 89.5) %.Histopathological analysis for FAs after FUAS treatment proved that FUAS could effectively induce irreversible coagulative necrosis of FA, and the tumor volume would gradually shrink in follow-up. FUAS was safe and effective to treat multiple FAs with good cosmesis.Key pointsThis study was the first study of detailed histopathological analysis for FAs after FUAS treatment.FUAS can effectively induce irreversible coagulative necrosis of fibroadenoma cells.FUAS ablation of multiple fibroadenomas is safe and effective.