化学交换饱和转移（CEST）已被探索用于区分前列腺癌（PCa）患者的肿瘤和良性组织。使用超高场强，例如7特斯拉（T），可以增加谱分辨率和灵敏度，从而允许选择性检测3.5ppm的酰胺质子转移（APT）和一组在2ppm谐振的化合物（即多胺/肌酸酐）。在已知存在局限性PCa并安排进行机器人辅助根治性前列腺切除术（RARP）的患者中研究了7T多池CEST分析前列腺和PCa的检测潜力。总共招募了12名患者进行前瞻性研究（平均年龄68.0岁，平均血清PSA 7.8）。分析了24个直径大于2毫米的病变。使用了7T T2加权成像（T2WI）和48个谱点CEST。患者接受了1.5/3T前列腺MRI以及镓-68特异性膜抗原（68 Ga-PSMA）PET / CT以确定单层CEST的位置。根据RARP后的组织病理学结果，在已知恶性区域和中央区以及外周区的良性区域中绘制了三个感兴趣区域（ROI）。这些区域被转换为CEST数据，从中计算出APT-和2ppm-CEST。使用Kruskal-Wallis检验计算了中央区，外周区和肿瘤之间CEST的统计学显著性。z-谱显示可检测到APT甚至在2ppm谐振的显著池中。该研究显示在中央区，周边区和肿瘤之间测试时APT水平存在差异趋势，但在2ppm水平没有差异（H（2）=4.8，P值=0.093和H（2）=0.86，P值=0.651，分别）。因此，我们最有可能使用CEST效应在前列腺中非侵入性地检测APT和胺/肌酸酐水平。在群体水平上，CEST显示周边区的APT水平高于中央区，但在肿瘤中未观察到APT和2ppm水平的差异。本文受版权保护，所有权利均归作者所有。

Chemical exchange saturation transfer (CEST) has been explored for differentiation between tumour and benign tissue in prostate cancer (PCa) patients. With ultra-high field strengths such as 7 Tesla (T), the increase of spectral resolution and sensitivity could allow for selective detection of amide proton transfer at 3.5ppm (APT) and a group of compounds that resonate at 2ppm (i.e. (poly)amines and/or creatine). The potential of 7T multipool CEST analysis of the prostate and the detection of PCa was studied in patients with proven localized PCa who were scheduled to undergo robot-assisted radical prostatectomy (RARP). Twelve patients were prospectively included, (mean age 68.0 years, mean serum-PSA 7.8). A total of 24 lesions >2mm were analysed. 7T T2-weighted imaging (T2WI) and 48 spectral points CEST were used. Patients received a 1.5/3T prostate MRI, and galium-68-prostate specific membrane antigen (68 Ga-PSMA)-PET/CT to determine location of the single-slice CEST. Based on the histopathological results after RARP, three regions of interest (ROI) were drawn in the T2WI from a known malignant zone and a benign zone in the central zone and peripheral zone. These areas were transposed to the CEST data from which the APT- and 2ppm-CEST were calculated. Statistical significance of the CEST between central zone, peripheral zone and tumour was calculated using a Kruskal-Wallis test. The z-spectra show that APT and even a distinct pool that resonate at 2ppm are detectable. This study showed a difference trend in the APT levels, but no difference in the 2ppm levels when tested between central zone, peripheral zone and tumour (H(2)=4.8, P-value=0.093 and H(2)=0.86, P-value=0.651, respectively). Thus to conclude, we could most likely detect APT and amines and/or creatine levels non-invasively in prostate using CEST effect. On group level, CEST showed a higher level of APT in peripheral zone versus central zone, however no differences of APT and 2ppm levels were observed in tumours.This article is protected by copyright. All rights reserved.