浸润瘤的T细胞是瘤免疫治疗中不可或缺的角色。在T细胞异质性的研究中取得了巨大进展。然而，关于不同癌症中浸润瘤T细胞的共同特征仍知之甚少。在本研究中，我们对15种癌症中的349,799个T细胞进行了泛癌症分析。结果表明，同一类型的T细胞在不同癌症中由特定的转录因子（TF）调控其表达模式。在癌症中，多种T细胞类型的转换路径是一致的。我们发现，与CD8+ T细胞相关的TF调控途径转化为终末分化的效应记忆（Temra）或疲劳（Tex）状态，与患者的临床分类有关。我们还观察到，浸润瘤T细胞之间的细胞间相互作用途径在所有癌症中都是普遍激活的，其中一些途径在特定细胞类型中特别调节信息传递。此外，在可变区和连接区基因方面，TCR的一致特征在不同癌症中被发现。总的来说，我们的研究揭示了不同癌症中浸润瘤T细胞的共同特点，并为有针对性的治疗开辟了未来途径。© 2023作者。

Tumor-infiltrating T cells are essential players in tumor immunotherapy. Great progress has been achieved in the investigation of T cell heterogeneity. However, little is well known about the shared characteristics of tumor-infiltrating T cells across cancers. In this study, we conduct a pan-cancer analysis of 349,799 T cells across 15 cancers. The results show that the same T cell types had similar expression patterns regulated by specific transcription factor (TF) regulons across cancers. Multiple T cell type transition paths were consistent in cancers. We found that TF regulons associated with CD8+ T cells transitioned to terminally differentiated effector memory (Temra) or exhausted (Tex) states were associated with patient clinical classification. We also observed universal activated cell-cell interaction pathways of tumor-infiltrating T cells in all cancers, some of which specifically mediated crosstalk in certain cell types. Moreover, consistent characteristics of TCRs in the aspect of variable and joining region genes were found across cancers. Overall, our study reveals common features of tumor-infiltrating T cells in different cancers and suggests future avenues for rational, targeted immunotherapies.© 2023 The Authors.