CD44和CD133是结直肠癌（CRC）干细胞标记物。CD44有着不同的异构体，具有不同的肿瘤学特性，如总CD44（CD44T）和变异CD44（CD44V）。这些标记物的临床意义仍不明确。我们以定量PCR检测60个结肠癌患者的CD44T/CD44V和CD133的mRNA水平，并阐明它们与临床病理因素的关联。 （1）CD44T和CD44V在原发性结肠肿瘤中的表达高于非癌性黏膜（p＜0.0001），而CD133的表达即使在非癌性黏膜中也有表达，且在肿瘤中有所降低（p=0.048）。 （2）CD44V表达与CD44T表达显著相关（R = 0.62，p＜0.0001），它们与原发性肿瘤中的CD133没有关联。 （3）CD44V / CD44T的表达在右结肠癌中显著高于左结肠癌（分别为p=0.035/p=0.012），而CD133的表达没有差异（p=0.20）。（4）在原发性肿瘤中，出乎意料的是，CD44V / CD44T / CD133 mRNA表达与侵袭性表型没有关联，而是与较不侵袭的淋巴结转移/远处转移表现显著相关（p=0.040 / p=0.039）。此外，与原发性肿瘤相比，肝转移中的CD44V和CD133表达均显著降低（分别为p=0.0005和p=0.0006）。我们对癌症干细胞标记物的转录本表达分析并未得出它们的表达可以代表原发性和转移性肿瘤的侵袭性表型，并且更多地代表了对干细胞标记物阳性的癌细胞的需求较少。 版权所有：©2023年Kaida等人。本文是根据创作共用署名许可证发布的开放获取文章，允许在任何媒介上不受限制地使用，分发和复制，前提是原始作者和出处得到了适当的认可。

CD44 and CD133 are stem cell markers in colorectal cancer (CRC). CD44 has distinctive isoforms with different oncological properties like total CD44 (CD44T) and variant CD44 (CD44V). Clinical significance of such markers remains elusive.Sixty colon cancer were examined for CD44T/CD44V and CD133 at mRNA level in a quantitative PCR, and clarified for their association with clinicopathological factors.(1) Both CD44T and CD44V showed higher expression in primary colon tumors than in non-cancerous mucosas (p<0.0001), while CD133 was expressed even in non-cancerous mucosa and rather decreased in the tumors (p = 0.048). (2) CD44V expression was significantly associated with CD44T expression (R = 0.62, p<0.0001), while they were not correlated to CD133 at all in the primary tumors. (3) CD44V/CD44T expressions were significantly higher in right colon cancer than in left colon cancer (p = 0.035/p = 0.012, respectively), while CD133 expression were not (p = 0.20). (4) In primary tumors, unexpectedly, CD44V/CD44T/CD133 mRNA expressions were not correlated with aggressive phenotypes, but CD44V/CD44T rather significantly with less aggressive lymph node metastasis/distant metastasis (p = 0.040/p = 0.039, respectively). Moreover, both CD44V and CD133 expressions were significantly decreased in liver metastasis as compared to primary tumors (p = 0.0005 and p = 0.0006, respectively).Our transcript expression analysis of cancer stem cell markers did not conclude that their expression could represent aggressive phenotypes of primary and metastatic tumors, and rather represented less demand on stem cell marker-positive cancer cells.Copyright: © 2023 Kaida et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.