小细胞肺癌（SCLC）是一种高度致命的恶性肿瘤，约占新诊断肺癌的15％。长链非编码RNA（lncRNA）可以通过与微小RNA（miRNA）的相互作用调节基因表达并促进肿瘤发生。然而，在SCLC中，有关lncRNA，miRNA和mRNA表达谱的研究还很有限。此外，与SCLC中的竞争性内源RNA（ceRNA）网络相关的差异表达的lncRNA，miRNA和mRNA的作用仍不清楚。在本研究中，我们首先对来自SCLC患者的六对SCLC肿瘤和相邻非癌组织进行了下一代测序（NGS）。总体上，在SCLC样本中发现29个lncRNA，48个miRNA和510个mRNA差异表达（|log2 [倍数变化] |> 1; P <0.05）。进行了生物信息学分析，预测并构建了lncRNA-miRNA-mRNA ceRNA网络，其中包括9个lncRNA，11个miRNA和392个mRNA。选择了ceRNA调节途径中的四个上调lncRNA和相关mRNA，并进行了定量PCR验证。此外，我们还研究了最上调lncRNA TCONS_00020615在SCLC细胞中的作用。我们发现，TCONS_00020615可能通过TCONS_00020615-hsa-miR-26b-5p-TPD52通路调节SCLC肿瘤发生。我们的研究对SCLC肿瘤和相邻非癌组织的lncRNA，miRNA和mRNA表达谱进行了全面分析。我们构建了ceRNA网络，这可能为SCLC的潜在调节机制提供新的证据。我们还发现，lncRNA TCONS_00020615可能调节SCLC的癌变发生。©2023年。作者（们）。

Small cell lung cancer (SCLC) is a highly lethal malignant tumor. It accounts for approximately 15% of newly diagnosed lung cancers. Long non-coding RNAs (lncRNAs) can regulate gene expression and contribute to tumorigenesis through interactions with microRNAs (miRNAs). However, there are only a few studies reporting the expression profiles of lncRNAs, miRNAs, and mRNAs in SCLC. Also, the role of differentially expressed lncRNAs, miRNAs, and mRNAs in relation to competitive endogenous RNAs (ceRNA) network in SCLC remain unclear.In the present study, we first performed next generation sequencing (NGS) with six pairs of SCLC tumors and adjacent non-cancerous tissues obtained from SCLC patients. Overall, 29 lncRNAs, 48 miRNAs, and 510 mRNAs were found to be differentially expressed in SCLC samples (|log2[fold change] |> 1; P < 0.05). Bioinformatics analysis was performed to predict and construct a lncRNA-miRNA-mRNA ceRNA network, which included 9 lncRNAs, 11 miRNAs, and 392 mRNAs. Four up-regulated lncRNAs and related mRNAs in the ceRNA regulatory pathways were selected and validated by quantitative PCR. In addition, we examined the role of the most upregulated lncRNA, TCONS_00020615, in SCLC cells. We found that TCONS_00020615 may regulate SCLC tumorigenesis through the TCONS_00020615-hsa-miR-26b-5p-TPD52 pathway.Our study provided the comprehensive analysis of the expression profiles of lncRNAs, miRNAs, and mRNAs of SCLC tumors and adjacent non-cancerous tissues. We constructed the ceRNA networks which may provide new evidence for the underlying regulatory mechanism of SCLC. We also found that the lncRNA TCONS_00020615 may regulate the carcinogenesis of SCLC.© 2023. The Author(s).