特应性皮炎（AD）是一种慢性炎症性皮肤疾病，广泛影响儿童和成人，会出现瘙痒、红斑、脱屑和干燥等症状。 萘醇是一种五环三萜，具有抗炎和抗微生物活性。 基于这些特性，萘醇在皮肤疾病的治疗效果方面一直受到积极研究。 在本研究中，我们旨在确定萘醇对AD的有效性。我们使用肿瘤坏死因子（TNF）-α / 干扰素（IFN）-γ刺激的角质-细胞和2,4-二硝基氯苯/螨抗原提取物（DFE）诱导的AD小鼠来确认其作用。萘醇通过减少促炎细胞因子和趋化因子的表达来抑制TNF-α / IFN-γ刺激的角质细胞活化，这些因子是通过激活信号转导分子，如转录因子-1、有丝分裂原活化蛋白激酶（p38和ERK）以及核因子κB介导的。口服萘醇抑制了耳组织中的表皮和真皮增厚以及免疫细胞浸润。萘醇也减少了血清中免疫球蛋白（Ig）E（总和DFE特异性）和IgG2a水平。萘醇降低了耳组织中T辅助（Th）2细胞因子、Th1细胞因子和促炎细胞因子的基因表达和蛋白分泌。这些结果表明，萘醇对AD相关反应具有抑制作用。因此，萘醇可能是AD的一种有前途的治疗药物。©2023作者。

Atopic dermatitis (AD) is a chronic inflammatory skin disease that affects from children to adults widely, presenting symptoms such as pruritus, erythema, scaling, and dryness. Lupeol, a pentacyclic triterpenoid, has anti-inflammatory and antimicrobial activities. Based on these properties, the therapeutic effects of lupeol on skin disorders have been actively studied. In the present study, we aimed to determine the effectiveness of lupeol on AD.We utilized tumor necrosis factor (TNF)-α/interferon (IFN)-γ-stimulated keratinocytes and 2, 4-dinitrochlorobenzene/Dermatophagoides farinae extract (DFE)-induced AD mice to confirm the action.Lupeol inhibited TNF-α/IFN-γ-stimulated keratinocytes activation by reducing the expressions of pro-inflammatory cytokines and chemokines which are mediated by the activation of signaling molecules such as signal transducer and activator of transcription 1, mitogen-activated protein kinases (p38 and ERK), and nuclear factor-κB. Oral administration of lupeol suppressed epidermal and dermal thickening and immune cell infiltration in ear tissue. Immunoglobulin (Ig) E (total and DFE-specific) and IgG2a levels in serum were also reduced by lupeol. The gene expression and protein secretion of T helper (Th) 2 cytokines, Th1 cytokines, and pro-inflammatory cytokine in ear tissue were decreased by lupeol.These results suggest that lupeol has inhibitory effects on AD-related responses. Therefore, lupeol could be a promising therapeutic agent for AD.© 2023. The Author(s).