lncRNA HOTAIR在乳腺癌组织中经常高表达，对乳腺癌的发展起着重要作用。在此，我们研究了lncRNA HOTAIR对乳腺癌细胞生物行为的影响及其分子机制。我们通过生物信息学方法研究了HOTAIR在乳腺癌中的水平及其临床病理特征。然后，我们通过qPCR，细胞计数试剂盒-8（CCK-8）测定，克隆形成实验，Transwell实验和细胞增殖，侵袭，迁移和凋亡的流式细胞术和细胞周期分析，评估了 HOTAIR和miRNA-1表达对乳腺癌细胞生物行为的影响。最后，通过荧光素酶报道验证了lncRNA HOTAIR / miR-1 / GOLPH3调控轴的靶基因。乳腺癌组织中HOTAIR的表达显著高于正常乳腺组织（P <0.05）。HOTAIR的沉默抑制了乳腺癌细胞的增殖，侵袭和迁移，促进了凋亡，并诱导了G1期阻滞（P <0.0001）。我们还通过荧光素酶报道实验证明miR-1是HOTAIR的靶标，GOLPH3是miR-1的靶标（P <0.001）。HOTAIR的表达在乳腺癌组织中显著升高。减少HOTAIR的表达抑制了乳腺癌细胞的增殖，侵袭和迁移，并促进了凋亡，其机制主要是lncRNA HOTAIR / miR-1 / GOLPH3调控轴对乳腺癌细胞生物行为的影响。©2023年，作者（们）在Federación de Sociedades Españolas de Oncología（FESEO）的独家许可下发表。

The lncRNA HOTAIR is frequently overexpressed in breast cancer tissues and plays an important role in the development of breast cancer. Here, we investigated the effect of the lncRNA HOTAIR on the biological behaviour of breast cancer cells and its molecular mechanism.We investigated the level of HOTAIR in breast cancer and its clinical pathological characteristics by bioinformatic methods. Then, we evaluated the effects of HOTAIR and miRNA-1 expression on the biological behaviour of breast cancer cells by qPCR, Cell Counting Kit-8 (CCK-8) assay, clonogenic assays, Transwell assay and flow cytometry for cell proliferation, invasion migration and apoptosis, and cell cycle analysis. Finally, the target genes of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis were validated by luciferase reports.The expression of HOTAIR in breast cancer tissues was significantly higher than that in normal breast tissues (P < 0.05). Silencing of HOTAIR suppressed cell proliferation, invasion and migration, promoted apoptosis and induced G1 phase block in breast cancer (P < 0.0001). We also verified that miR-1 is a target of HOTAIR and that GOLPH3 is a target of miR-1 by luciferase reporter assays (P < 0.001).The expression of HOTAIR was significantly elevated in breast cancer tissues. Reducing the expression of HOTAIR inhibited the proliferation, invasion and migration of breast cancer cells and promoted apoptosis, and the mechanism was mainly the effect of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis on the biological behaviour of breast cancer cells.© 2023. The Author(s), under exclusive licence to Federación de Sociedades Españolas de Oncología (FESEO).