蚕蛹蛋白（Bombyx mori protein，SPP）是一种潜在的治疗生物活性物质，具有对乳腺癌、肝癌和胃癌的抗肿瘤活性。本研究旨在研究SPP对结肠癌裸鼠的抗肿瘤效果。通过皮下肿瘤形成方法，在裸鼠体内验证了SPP对结肠癌的治疗效果。结果显示SPP对肿瘤细胞具有细胞毒性作用。SPP可通过降低肝脏氧化应激和调节血清炎症水平（通过降低TNF-α和IL-2水平，增加INF-γ水平）来保护裸鼠的肝脏功能。此外，SPP降低了Ki-67蛋白，增强了cleaved caspase-3蛋白，降低了Vimentin蛋白，增强了E-cadherin蛋白。这些发现表明，SPP的抗肿瘤活性可能通过减轻炎症、抑制肿瘤增殖和转移以及诱导癌细胞凋亡来实现。未来，SPP可以作为一种抗癌药物使用，为结肠癌的治疗提供可能的新药物来源。版权所有 © 2023 Zhou, Ji, Wang, Guo, Zhao and Yan.

Silkworm pupa (bombyx mori) protein (SPP) is a potential therapeutic bioactive substance that has anti-tumor activity against breast, liver, and gastric cancers. The aim of this study was to investigate the antitumor effect of SPP on colon cancer nude mice. Using a subcutaneous tumor formation method, we validated the therapeutic effect of SPP on colon cancer nude mice in vivo. Results showed that SPP was cytotoxic to tumor cells. SPP could protect the liver of the nude mice by lowering hepatic oxidative stress and regulating serum inflammation levels by decreasing TNF-α and IL-2 levels while in-creasing INF-γ levels. In addition, diminished Ki-67 protein, enhanced cleaved caspase-3 protein, di-minished Vimentin, enhanced E-cadherin. These findings suggested that SPP's antitumor activity may be achieved by reducing inflammation, inhibiting tumor proliferation and metastasis, and inducing apoptosis in cancer cells. In the future, SPP could be used as an anticancer drug, potentially providing a new source of drugs for the treatment of colon cancer.Copyright © 2023 Zhou, Ji, Wang, Guo, Zhao and Yan.