胎盘特异性基因1（PLAC1）是一种癌胚诊断抗原，正常组织中仅在胎盘滋养细胞和睾丸生精细胞中表达，但在多种实体肿瘤中过度表达。目前对于PLAC1在白血病中的表达缺乏研究。我们调查了急性髓系白血病（AML）和急性淋巴细胞白血病（ALL）中PLAC1的表达。在本研究中，我们使用定量实时PCR检测了AML（n=31）和ALL（n=31）新诊断患者的外周血和骨髓单个核细胞中PLAC1基因的表达模式。正常受试者（n=17）被作为对照组。我们还使用蛋白质印迹法检测了样本中PLAC1蛋白的表达。 我们的数据表明，与正常样本相比，AML和ALL中的PLAC1转录本分别增加了2.7倍和2.9倍。所有研究的正常受试者的PLAC1转录本表达完全为阴性。与正常样本相比，ALL中PLAC1 mRNA的表达水平在统计上显著增加（p=0.038）。然而，与正常受试者相比，AML中PLAC1的相对mRNA表达并不显著（p=0.848）。此外，AML亚型中PLAC1的相对mRNA表达也没有统计学显著性（p=0.756）。PLAC1基因表达在人口学临床和副临床参数方面没有差异。蛋白质印迹法证实了ALL和AML样本中PLAC1的表达。 考虑到急性白血病中的PLAC1表达特征，PLAC1可能是一种未来需要进一步研究的白血病潜在标志物。 版权所有©2023 Avicenna研究所。

Placenta-specific 1 (PLAC1) is one of the cancer-testis-placenta antigens that has no expression in normal tissue except placenta trophoblast and testicular germ cells, but is overexpressed in a variety of solid tumors. There is a lack of studies on the expression of PLAC1 in leukemia. We investigated expression of PLAC1 in Acute Myeloid Leukemia (AML) and Acute Lymphoblastic Leukemia (ALL).In this study, we investigated expression pattern of PLAC1 gene in peripheral blood and bone marrow mononuclear cells of newly-diagnosed patients with AML (n=31) and ALL (n=31) using quantitative real-time PCR. Normal subjects (n=17) were considered as control. The PLAC1 protein expression in the samples were also detected using western blotting.Our data demonstrated that PLAC1 transcripts had 2.7 and 2.9 fold-change increase in AML and ALL, respectively, compared to normal samples. PLAC1 transcript expression was totally negative in all studied normal subjects. Level of PLAC1 mRNA expression in ALL statistically increased compared to normal samples (p=0.038). However, relative mRNA expression of PLAC1 in AML was not significant in comparison to normal subjects (p=0.848). Furthermore, relative mRNA expression of PLAC1 in AML subtypes was not statistically significant (p=0.756). PLAC1 gene expression showed no difference in demographical clinical and para-clinical parameters. Western blotting confirmed expression of PLAC1 in the ALL and AML samples.Considering PLAC1 expression profile in acute leukemia, PLAC1 could be a potential marker in leukemia which needs complementary studies in the future.Copyright© 2023 Avicenna Research Institute.