柑橘果皮多酚具有明显的抗炎活性。然而，其对溃疡性结肠炎的潜在机制尚未阐明。本研究的目的是研究柑橘果皮多酚的抗炎效果和作用机制。采用大孔吸附树脂对柑橘果皮多酚进行浓缩，并分离成水溶性柑橘多酚和酯溶性柑橘果皮多酚。然后将这些提取物以300 mg/kg▪bw的剂量灌胃给予经硫酸戊糖诱导的急性结肠炎小鼠进行为期14天的治疗。高效液相色谱结果显示提取物含有黄酮酮类、黄酮类和酚酸类化合物。与硫酸戊糖组相比，总柑橘果皮多酚、水溶性柑橘多酚和酯溶性柑橘果皮多酚显著改善了结肠炎症状的严重程度。此外，柑橘果皮多酚减少了髓过氧化酶的活性，降低了肿瘤坏死因子-α和白介素-6的分泌，并增加了白介素-10的产生。同时，总柑橘果皮多酚、水溶性柑橘多酚和酯溶性柑橘果皮多酚有效阻断了核因子-kappa B的激活。这些结果表明，柑橘果皮多酚通过抑制炎症因子分泌和抑制核因子-kappa B通路的激活来缓解小鼠的溃疡性结肠炎。© 2023 The Authors. Published by Elsevier Ltd.

Citrus peel polyphenols have possess the distinct anti-inflammatory activities. However, its underlying mechanism on ulcerative colitis have not been elucidated. The aim of this research was to investigate the anti-inflammatory effect and action mechanisms of citrus peel polyphenols. Total citrus peel polyphenols were concentrated using macroporous resins and separated into water-soluble citrus polyphenols and ester-soluble citrus peel polyphenols. These extracts were then gavaged to acute colitis mice induced by dextran sulfate sodium for 14 days using a dose of 300 mg/kg▪bw. High performance liquid chromatography results showed that the extracts contained flavanones, flavonoids, and phenolic acids. Compared to the dextran sulfate sodium group, total citrus peel polyphenols, water-soluble citrus polyphenols, and ester-soluble citrus peel polyphenols significantly ameliorated the severity of colitis symptoms. Additionally, citrus peel polyphenols reduced the activity of myeloperoxidase, lowered secretion of tumor necrosis factor-α and interleukin-6, and increased interleukin-10. Meanwhile, total citrus peel polyphenols, water-soluble citrus polyphenols, and ester-soluble citrus peel polyphenols effectively blocked the activation of the nuclear factor-kappa B. These results demonstrated that citrus peel polyphenols alleviated ulcerative colitis in mice by damping pro-inflammatory cytokine secretion and suppressing the nuclear factor-kappa B pathway activation.© 2023 The Authors. Published by Elsevier Ltd.