分子伴侣蛋白，如热休克蛋白（HSPs），已经引起了人们的关注，因为它们参与了癌症恶性事件，并有潜在的治疗靶点和肿瘤治疗的生物标记。此外，伴侣蛋白的突变会显著影响癌症的风险和预后。生物信息学是一种特别有用的方法，可以作为数据的即时临床应用的实际考虑来开发生物标记。如今，许多大规模的癌症基因组数据库和门户都是公开可用的，包括国际癌症基因组联盟（ICGC）、癌症基因组图谱（TCGA，现已更名为基因组数据共享库（GDC））、癌症体细胞突变目录（COSMIC）和癌细胞系百科全书（CCLE）。参考这些数据库，已经公开了先进的网络门户，包括cBioPortal、人蛋白质图谱（HPA）、Kaplan-Meier（KM）绘图器、基因表达谱互动分析2（GEPIA2）、癌症中药敏感性基因组学（GDSC）和依赖力地图（DepMap）。在这里，我们整理了这些数据库和门户，以澄清当前癌症研究中有哪些可用和有用的内容，并提供使用HPA、KM绘图器和GEPIA2进行癌症患者伴侣基因研究的协议。利用这些门户将揭示肿瘤亚型特异性伴侣基因高表达与患者预后之间的相关性。我们的方案对提高人们对伴侣蛋白的系统意识，寻找诊断和预后的新生物标记以及新的抗癌药物靶点具有实用价值。© 2023作者，Springer Science+Business Media, LLC， Springer Nature的独家许可证下。

Molecular chaperones, such as heat shock proteins (HSPs), have attracted attention as molecules involved in malignant events in cancers and are potential therapeutic targets and biomarkers for tumor therapy. Furthermore, mutations in chaperones can significantly impact cancer risk and prognosis. Bioinformatics is a particularly useful method for developing biomarkers as a practical consideration for the immediate clinical application of data. Many large-scale databases and portals on cancer genome are nowadays publicly available, including the International Cancer Genome Consortium (ICGC); The Cancer Genome Atlas (TCGA), renamed as Genomic Data Commons (GDC); Catalogue of Somatic Mutations in Cancer (COSMIC); and Cancer Cell Line Encyclopedia (CCLE). Referring to these databases, advanced web portals are publicized, including cBioPortal, Human Protein Atlas (HPA), Kaplan-Meier (KM) plotter, Gene Expression Profiling Interactive Analysis 2 (GEPIA2), Genomics of Drug Sensitivity in Cancer (GDSC), and Dependency Map (DepMap). Here, we assemble these databases and portals to clarify what is available and useful for current cancer research and provide protocols to utilize the HPA, KM plotter, and GEPIA2 for studies on chaperone genes in cancer patients. Utilizing these portals will reveal the correlation between tumor subtype-specific high expression of chaperone genes and patient prognosis. Our protocols are useful to increase systematic awareness of chaperones and find new biomarkers for diagnosis and prognosis and new targets for anticancer drugs.© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.